EXPERIMENTAL-INFECTION OF NEONATAL CALVES WITH NEUROVIRULENT BOVINE HERPESVIRUS TYPE-1.3
EXPERIMENTAL-INFECTION OF NEONATAL CALVES WITH NEUROVIRULENT BOVINE HERPESVIRUS TYPE-1.3
复制标题
DOI：
10.1177/030098589403100309
复制
发表时间：
1994-05-01
影响因子：
2.4
通讯作者：
SCHULTHEISS, PC
中科院分区：
文献类型：
--
作者：
BELKNAP, EB;COLLINS, JK;SCHULTHEISS, PC
牛疱疹病毒 BHV-1.3 会引起犊牛脑炎，而 BHV-1.1 则会引起呼吸道疾病。通过鼻内雾化法给三头剥夺初乳的小牛和两只喂养初乳的小牛接种 BHV-1.3。通过鼻内雾化接种 BHV-1.1 的两只初乳剥夺的小牛。接种 BHV-1.3 的小牛表现出严重的脑炎，伴有轻微的呼吸道病变，而接种 BHV-1.1 的小牛则表现出严重的呼吸道病变，但没有神经系统疾病的临床症状。饲喂含有病毒中和抗体的初乳的小牛可以预防神经系统疾病。接种初乳的 BHV-1.3 犊牛虽然被感染，但并未发病；接种后 10-13 天，病毒在呼吸道分泌物中排出，类似于受感染的缺乏初乳的犊牛。接种后 60 天给予地塞米松后，BHV-1.3 从一头初乳喂养的小牛身上的潜伏状态重新激活。对三头接种 BHV-1.3 的不接种初乳的小牛进行组织病理学检查，结果显示存在严重的脑炎病变。两只接种 BHV-1.1 的小牛中的一只出现一处局灶性脑炎病变。从缺乏初乳的 BHV-1.3 接种小牛和一头 BHV-1.1 接种小牛的脑组织中分离出病毒。在来自初乳剥夺的 BHV-1.3 接种小牛的神经元中观察到 BHV-1 抗原的免疫组织化学染色。
A type of bovine herpesvirus, BHV-1.3, causes encephalitis in calves, whereas BHV-1.1 causes respiratory disease. Three colostrum-deprived calves and two colostrum-fed calves were inoculated with BHV-1.3 by intranasal aerosolization. Two colostrum-deprived calves were inoculated with BHV-1.1 by intranasal aerosolization. BHV-1.3-inoculated calves demonstrated severe encephalitis with minimal respiratory lesions, and BHV-1.1-inoculated calves demonstrated severe respiratory lesions and no clinical signs of neurologic disease. Calves fed colostrum that contained virus neutralizing antibodies were protected against neurologic disease. Colostrum-fed BHV-1.3-inoculated calves did not develop disease although they did become infected; virus was shed in respiratory secretions for 10-13 days postinoculation, similar to infected colostrum-deprived calves. BHV-1.3 was reactivated from a latent state from one colostrum-fed calf after administration of dexamethasone 60 days postinoculation. Histopathologic examination of the three colostrum-deprived BHV-1.3-inoculated calves revealed severe lesions of encephalitis. One of the two BHV-1.1-inoculated calves had one focal lesion of encephalitis. Virus was isolated from brain tissue of colostrum-deprived BHV-1.3-inoculated calves and from one BHV-1.1-inoculated calf. Immunohistochemical staining for BHV-1 antigen was observed in neurons from the colostrum-deprived BHV-1.3-inoculated calves.