Associations between Co-Detected Respiratory Viruses in Children with Acute Respiratory Infections
Associations between Co-Detected Respiratory Viruses in Children with Acute Respiratory Infections
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DOI：
10.7883/yoken.67.469
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发表时间：
2014-11-01
影响因子：
2.2
通讯作者：
Iritani, Nobuhiro
中科院分区：
文献类型：
--
作者：
Kaida, Atsushi;Kubo, Hideyuki;Iritani, Nobuhiro
病毒是幼儿急性呼吸道感染（阿里斯）的主要病原体。尽管呼吸道病毒联合检测很常见，但日本从未对联合检测到的病毒组合进行过分析。使用多重实时PCR对2010年1月至2011年12月期间在日本大坂市采集的1，044份儿童（患者年龄< 6岁）ARI标本进行了19种呼吸道病毒或亚型的调查。总共有891份标本（85.3%）为病毒阳性（检出1，414种病毒），其中388份病毒阳性标本（43.5%，388/891）为多种病毒阳性。在0-35个月的儿童中，多重/总呼吸道病毒阳性标本的比例较高。统计学分析显示，人博卡病毒1型和人腺病毒是同步共检测的。另一方面，1型人副流感病毒（HPIV-1）与HPIV-3、HPIV-3与人偏肺病毒（hMPV）、hMPV与呼吸道合胞病毒A（RSV A）、hMPV与甲型流感病毒（H1 N1）2009（FLUA（H1 N1）2009）、RSV A与RSV B以及人鼻病毒和FLUA（H1 N1）2009的共检测是排他性的。这些结果表明，幼儿（
Viruses are the major etiological agents of acute respiratory infections (ARIs) in young children. Although respiratory virus co-detections are common, analysis of combinations of co-detected viruses has never been conducted in Japan. Nineteen respiratory viruses or subtypes were surveyed using multiplex real-time PCR on 1,044 pediatric (patient age < 6 years) ARI specimens collected in Osaka City, Japan between January 2010 and December 2011. In total, 891 specimens (85.3%) were virus positive (1,414 viruses were detected), and 388 of the virus-positive specimens (43.5%, 388/891) were positive for multiple viruses. The ratio of multiple/total respiratory virus-positive specimens was high in children aged 0-35 months. Statistical analyses revealed that human bocavirus 1 and human adenovirus were synchronously co-detected. On the other hand, co-detections of human parainfluenza virus type 1 (HPIV-1) with HPIV-3, HPIV-3 with human metapneumovirus (hMPV), hMPV with respiratory syncytial virus A (RSV A), hMPV with influenza virus A (H1N1) 2009 (FLUA (H1N1) 2009), RSV A with RSV B, and human rhinovirus and FLUA (H1N1) 2009 were exclusive. These results suggest that young children (