METHODS FOR DETERMINING THE PROLIFERATION KINETICS OF CELLS BY MEANS OF 5‐BROMODEOXYURIDINE
METHODS FOR DETERMINING THE PROLIFERATION KINETICS OF CELLS BY MEANS OF 5‐BROMODEOXYURIDINE
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5-溴脱氧尿苷测定细胞增殖动力学的方法
DOI：
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发表时间：
1977
期刊：
影响因子：
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通讯作者：
K. Detzer
中科院分区：
文献类型：
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作者：
F. Pera;P. Mattias;K. Detzer
用5-溴脱氧尿苷（BUdR）处理细胞后，完全BUdR标记的间期核的百分比越大，BUdR处理时间越长。用荧光染料33258 Hoechst和Giemsa染色后，标记效果可见。本文提出了各种公式和列线图，用这些公式和列线图，可以通过检查一种制剂或比较几种制剂来确定S期细胞的百分比、S期的持续时间和整个细胞周期。使用田鼠的细胞培养物对该方法进行了检测，并与3 H-胸苷标记后的放射自显影方法进行了比较。
After treatment of cells with 5‐bromodeoxyuridine (BUdR), the percentage of completely BUdR‐labelled interphase nuclei is greater the longer the BUdR treatment. The labelling effect is visible after staining with the fluorochrome 33258 Hoechst and with Giemsa. Various formulae and a nomogram are presented by means of which the percentage of cells in S period, duration of the S period and the whole cell cycle can be determined by examination of a single preparation or by comparison of several preparations. The methods are tested using cell cultures of Microtus agrestis and compared with autoradiographic methods after labelling with 3H‐thymidine.