Analysis of organ physiology in transgenic mice.
Analysis of organ physiology in transgenic mice.
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DOI：
10.1152/ajpcell.2000.279.1.c1
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发表时间：
2000-07
期刊：
影响因子：
--
通讯作者：
Shobha Rao;A. Verkman
中科院分区：
文献类型：
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作者：
Shobha Rao;A. Verkman
基因缺失和人类疾病的转基因小鼠模型的日益增加的可用性已经要求开发创造性的方法来表征小鼠表型。小鼠由于其体积小、习性和无法描述临床症状而对表型分析提出了独特的挑战。本文综述了研究小鼠器官生理学的策略，重点是心血管，肺，肾，胃肠道和神经行为系统。一般关注评价小鼠表型研究进行了讨论。监测和麻醉方法进行审查，重点是无创和侵入性程序的可行性和局限性，以监测生理参数，做插管，并进行外科手术。表型研究的例子被引用来证明测量方法的实际应用和局限性。表型分析方法的剧目在这里审查应该是有用的研究人员参与或考虑使用小鼠模型。
The increasing availability of transgenic mouse models of gene deletion and human disease has mandated the development of creative approaches to characterize mouse phenotype. The mouse presents unique challenges to phenotype analysis because of its small size, habits, and inability to verbalize clinical symptoms. This review describes strategies to study mouse organ physiology, focusing on the cardiovascular, pulmonary, renal, gastrointestinal, and neurobehavioral systems. General concerns about evaluating mouse phenotype studies are discussed. Monitoring and anesthesia methods are reviewed, with emphasis on the feasibility and limitations of noninvasive and invasive procedures to monitor physiological parameters, do cannulations, and perform surgical procedures. Examples of phenotype studies are cited to demonstrate the practical applications and limitations of the measurement methods. The repertoire of phenotype analysis methods reviewed here should be useful to investigators involved in or contemplating the use of mouse models.