C-type lectins on macrophages participate in the immunomodulatory response to Fasciola hepatica products
C-type lectins on macrophages participate in the immunomodulatory response to Fasciola hepatica products
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DOI：
10.1111/j.1365-2567.2011.03449.x
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发表时间：
2011-07-01
期刊：
影响因子：
6.4
通讯作者：
Masih, Diana T.
中科院分区：
文献类型：
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作者：
Guasconi, Lorena;Serradell, Marianela C.;Masih, Diana T.
肝片吸虫释放排泄分泌产物（FhESP），这些寄生虫产物中存在的碳水化合物具有免疫调节特性。FhESP与先天免疫细胞如巨噬细胞的相互作用在感染的早期阶段至关重要。在这项工作中，我们观察到在体外用FhESP刺激的幼稚BALB/c小鼠的腹腔巨噬细胞呈现：增加的精氨酸酶活性以及精氨酸酶I表达，以及高水平的转化生长因子-β和白细胞介素-10。在感染小鼠的腹膜中也观察到类似的巨噬细胞群体。当巨噬细胞与甘露聚糖、抗甘露糖受体、海带多糖或抗Dectin-1预孵育，然后用FhESP刺激时，观察到上述免疫调节作用的部分抑制。此外，我们观察到在感染前腹腔注射甘露聚糖或海带多糖的小鼠中获得的巨噬细胞中这些作用的部分抑制。两者合计，这些结果表明，参与至少两个C型凝集素受体，甘露糖受体和Dectin-1，在FhESP与巨噬细胞的相互作用，这使得这种寄生虫诱导对这些重要的先天免疫细胞的免疫调节作用，并可能构成一个关键的事件，延长其在宿主中的生存。
P>Fasciola hepatica releases excretory-secretory products (FhESP), and immunomodulatory properties have been described for the carbohydrates present in these parasite products. The interaction of FhESP with the innate immune cells, such as macrophages, is crucial in the early stage of infection. In this work we observed that peritoneal macrophages from naive BALB/c mice stimulated in vitro with FhESP presented: an increased arginase activity as well as Arginase I expression, and high levels of transforming growth factor-beta and interleukin-10. A similar macrophage population was also observed in the peritoneum of infected mice. A partial inhibition of the immunomodulatory effects described above was observed when macrophages were pre-incubated with Mannan, anti-mannose receptor, Laminarin or anti-Dectin-1, and then stimulated with FhESP. In addition, we observed a partial inhibition of these effects in macrophages obtained from mice that were intraperitoneally injected with Mannan or Laminarin before being infected. Taken together, these results suggest the participation of at least two C-type lectin receptors, mannose receptor and Dectin-1, in the interaction of FhESP with macrophages, which allows this parasite to induce immunoregulatory effects on these important innate immune cells and may constitute a crucial event for extending its survival in the host.