TRANSLATION OF TOBACCO RATTLE VIRUS RNAS INVITRO - 4 PROTEINS FROM 3 RNAS
TRANSLATION OF TOBACCO RATTLE VIRUS RNAS INVITRO - 4 PROTEINS FROM 3 RNAS
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DOI：
10.1016/0042-6822(79)90337-4
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发表时间：
1979-01-01
期刊：
影响因子：
3.7
通讯作者：
PELHAM, HRB
中科院分区：
文献类型：
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作者：
PELHAM, HRB
烟草脆裂病毒利塞和PRN株的RNA在核酸酶处理的网织红细胞裂解物中翻译。对于这两种菌株，RNA-1编码两种分子量为170，000和120，000的蛋白质。添加1-2 mM Mg 2+增加了较大产物的产率，显然是通过促进在编码较小蛋白质的区域末端的泄漏终止密码子的通读。酵母抑制tRNA的实验表明这不是UAG或UAA密码子。RNA-2编码与外壳蛋白大小相同的蛋白质。MW 30，000的产物由第三RNA（RNA-3）编码，在PRN TRV的情况下，第三RNA具有550，000的MW，并且可以通过凝胶电泳与RNA-2分离。所有3种RNA的翻译均被m7 GTP抑制。RNA-1还指导低水平的m7 GTP抗性合成30，000-道尔顿蛋白，该蛋白似乎与RNA-3产物相同，并且RNA-3可能对应于RNA-1的3“末端。因此，烟草脆裂病毒和烟草花叶病毒的非外壳蛋白的合成策略似乎是相似的。
RNA from the Lisse and PRN strains of tobacco rattle virus were translated in nuclease-treated reticulocyte lysates. With both strains, RNA-1 coded for two proteins of MW 170,000 and 120,000. Addition of 1-2 mM Mg2+ increased the yield of the larger product, apparently by facilitating readthrough of a leaky termination codon at the end of the region coding for the smaller protein. Experiments with yeast suppressor tRNA indicate that this is not a UAG or UAA codon. RNA-2 coded for a protein of the same size as coat protein. A product of MW 30,000 was coded by a 3rd RNA (RNA-3) which in the case of PRN TRV had a MW of 550,000 and could be separated from RNA-2 by gel electrophoresis. Translation of all 3 RNA was inhibited by m7GTP. RNA-1 also directed a low level of m7GTP-resistant synthesis of a 30,000-dalton protein which appeared to be identical to the RNA-3 product, and RNA-3 probably corresponds to the 3'' end of RNA-1. Thus, the strategies of synthesis of the non-coat proteins of tobacco rattle and tobacco mosaic viruses appear to be similar.