Non-pneumococcal mitis-group streptococci confound detection of pneumococcal capsular serotype-specific loci in upper respiratory tract
Non-pneumococcal mitis-group streptococci confound detection of pneumococcal capsular serotype-specific loci in upper respiratory tract
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DOI：
10.7717/peerj.97
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发表时间：
2013-06-25
期刊：
影响因子：
2.7
通讯作者：
Beall, BernardW.
中科院分区：
文献类型：
--
作者：
Carvalho, Maria da Gloria;Pimenta, Fabiana C.;Beall, BernardW.
我们进行了基于培养和PCR的检测，从肯尼亚农村和城市收集的携带标本中进行肺炎球菌鉴定和血清分型。来自237名健康儿童的鼻咽样本（98.7%）与st的参考cmPCR扩增子，而来自lytA阴性样本的cmPCR扩增子通常更趋异。对56种A-OP和56种A-NP的单独测试显示，来自A-NP/OP的阳性cmPCR结果中有94%来自OP微生物群。相比之下，A-NP产生的肺炎球菌分离株比A-OP多>2倍。与A-OP相比，C-NP和A-NP中经验证和疑似的非肺炎球菌cmPCR血清型/血清群似乎相对罕见。我们的研究结果表明，非肺炎球菌的物种可以混淆，由于进化上保守的基因最有可能参与表面多糖结构的生物合成的特异性PCR和其他基于序列的测定。
We performed culture-based and PCR-based tests for pneumococcal identification and serotyping from carriage specimens collected in rural and urban Kenya. Nasopharyngeal specimens from 237 healthy children 98.7%) with the reference cmPCR amplicon for the st, while cmPCR amplicons from lytA-negative specimens were generally more divergent. Separate testing of 56 A-OPs and 56 A-NPs revealed that similar to 94% of the positive cmPCR results from A-NP/OPs were from OP microbiota. In contrast, A-NPs yielded >2-fold more pneumococcal isolates than A-OPs. Verified and suspected non-pneumococcal cmPCR serotypes/serogroups appeared to be relatively rare in C-NPs and A-NPs compared to A-OPs. Our findings indicate that non-pneumococcal species can confound serotype-specific PCR and other sequence-based assays due to evolutionarily conserved genes most likely involved in biosynthesis of surface polysaccharide structures.