Genotoxicity and reactive oxygen species production induced by magnetite nanoparticles in mammalian cells
Genotoxicity and reactive oxygen species production induced by magnetite nanoparticles in mammalian cells
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DOI：
10.2131/jts.38.503
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发表时间：
2013-06-01
影响因子：
2
通讯作者：
Yagi, Takashi
中科院分区：
文献类型：
--
作者：
Kawanishi, Masanobu;Ogo, Sayaka;Yagi, Takashi
我们研究了磁铁矿纳米颗粒（初级粒径：10 nm）对人A549和中国仓鼠卵巢（CHO）AA 8细胞的遗传毒性。在200 μ g/ml（34 μ g/cm（2））的剂量下，用颗粒处理6小时，A549和CHO AA 8细胞中的微核（MN）频率分别增加至5.2%和5.0%，并呈剂量依赖性。在A549细胞中，用纳米颗粒（2 μ g/ml）处理1小时诱导H2 AX磷酸化，这提示DNA双链断裂（DSB）。用2 μ g/ml（0.34 μ g/cm（2））磁铁矿处理CHO AA 8细胞1小时，姐妹染色单体交换（SCE）频率比对照水平高5倍。我们检测到活性氧（ROS）在CHO细胞处理的颗粒。这些发现表明，磁铁矿纳米颗粒诱导哺乳动物细胞中的ROS，导致直接或间接诱导DSB，随后发生包括MN和SCE的致染色体断裂事件。
We examined the genotoxicity of magnetite nanoparticles (primary particle size: 10 nm) on human A549 and Chinese hamster ovary (CHO) AA8 cells. Six hours' treatment with the particles dose-dependently increased the frequency of micronuclei (MN) in the A549 and CHO AA8 cells up to 5.2% and 5.0% at a dose of 200 mu g/ml (34 mu g/cm(2)), respectively. In A549 cells, treatment with the nanoparticles (2 mu g/ml) for 1 hr induced H2AX phosphorylation, which is suggestive of DNA double strand breaks (DSB). Treating CHO AA8 cells with 2 mu g/ml (0.34 mu g/cm(2)) magnetite for 1 hour resulted in a five times higher frequency of sister chromatid exchange (SCE) than the control level. We detected reactive oxygen species (ROS) in CHO cells treated with the particles. These findings indicate that magnetite nanoparticles induce ROS in mammalian cells, leading to the direct or indirect induction of DSB, followed by clastogenic events including MN and SCE.