Secretion of follicle-stimulating hormone and production of inhibin are reciprocally related.
Secretion of follicle-stimulating hormone and production of inhibin are reciprocally related.
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促卵泡激素的分泌和抑制素的产生是相互相关的。
DOI：
10.1073/pnas.84.13.4631
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发表时间：
1987
影响因子：
11.1
通讯作者：
Guillemin,R
中科院分区：
文献类型：
--
作者：
Ying,SY;Czvik,J;Becker,A;Ling,N;Ueno,N;Guillemin,R
用抗[Tyr30]抑制素α链-(1-30)复制的抗血清，用放射免疫法测定了去卵巢、雌激素处理的未成熟大鼠颗粒细胞和正常动物支持细胞中抑制素的产生。在这些细胞的废液中检测到的免疫反应抑制素的量与细胞的密度和外源添加的卵泡刺激素(FSH)的浓度成正比。在雌激素前体雄烯二酮(10(-7)M)存在的情况下，FSH，而不是黄体生成素，在颗粒细胞培养2天中产生剂量依赖性的抑制素增加。在没有雌激素前体的情况下，观察到对FSH的反应中抑制素的产生类似，但略有减少。在没有雄烯二酮的情况下，外源添加的雌激素增强了FSH介导的抑制素的释放。雄烯二酮和雌二醇对培养的Sertoli细胞的抑制素产生均无影响。一种根据体外生物测定分离的纯抑制素的制剂，其化学特性是特异性地抑制血清FSH，但不能抑制黄体生成素，当它被注射(每次24微克，两次注射)给急性去卵巢大鼠时。因此，颗粒细胞和支持细胞分泌的抑制素特异性地抑制垂体FSH的分泌，而FSH是这些性腺细胞产生抑制素的主要原因，就像经典的负反馈关系一样。
The production of inhibin in cultured granulosa cells from immature hypophysectomized, estrogen-treated rats and Sertoli cells from normal animals was determined by a specific radioimmunoassay using an antiserum against a synthetic replicate of [Tyr30]inhibin alpha-chain-(1-30). The amount of immunoreactive inhibin detected in the spent media of these cells is in proportion to the density of cells plated and the concentration of exogenously added follicle-stimulating hormone (FSH). In the presence of the estrogen precursor androstenedione (10(-7) M), FSH, but not luteinizing hormone, produced a dose-dependent increase in inhibin during 2-day culture of granulosa cells. In the absence of the estrogen precursor, similar but somewhat diminished inhibin production in responding to FSH was observed. Exogenously added estrogen potentiated the FSH-mediated release of inhibin in the absence of androstenedione. Neither androstenedione nor estradiol added to the cultured Sertoli cells had effect on inhibin production. A preparation of pure inhibin isolated on the basis of an in vitro bioassay and characterized chemically specifically suppressed serum FSH but not luteinizing hormone, when it was injected (24 micrograms per injection, two injections) into acutely ovariectomized rats. Thus, inhibin secreted by the granulosa and Sertoli cells specifically suppresses the secretion of pituitary FSH, and in turn FSH is primarily responsible for the inhibin production in these gonadal cells, as in a classical negative-feedback relationship.