SarA influences the sporulation and secondary metabolism in Streptomyces coelicolor M145
SarA influences the sporulation and secondary metabolism in Streptomyces coelicolor M145
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SarA 影响天蓝色链霉菌 M145 的孢子形成和次生代谢
DOI：
10.1111/j.1745-7270.2008.00466.x
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发表时间：
2008-10-01
影响因子：
3.7
通讯作者：
Ding, Xiaoming
中科院分区：
文献类型：
--
作者：
Ou, Xijun;Zhang, Bo;Ding, Xiaoming
丝状菌链霉菌具有复杂的生活史，包括形态分化和次生代谢。天蓝色链霉菌M145中存在一个膜蛋白基因sarA（sco 4069），即产孢和抗生素产生相关基因A。该基因产物在链霉菌中高度保守，没有特征性的功能结构域。与野生型M145相比，sarA突变体加速了孢子形成，并显著降低了放线菌紫素和十一烷基灵菌红素的产量。逆转录-聚合酶链反应分析表明，SarA通过控制actII-orf 4和redZ信使RNA的丰度来影响抗生素的产生。
The filamentous bacteria Streptomyces exhibit a complex life cycle involving morphological differentiation and secondary metabolism. A putative membrane protein gene sarA (sco4069), sporulation and antibiotic production related gene A, was partially characterized in Streptomyces coelicolor M145. The gene product had no characterized functional domains and was highly conserved in Streptomyces. Compared with the wild-type M145, the sarA mutant accelerated sporulation and dramatically decreased the production of actinorhodin and undecylprodigiosin. Reverse transcription-polymerase chain reaction analysis showed that SarA influenced antibiotic production by controlling the abundance of actII-orf4 and redZ messenger RNA.