Chronic methamphetamine regulates the expression of MicroRNAs and putative target genes in the nucleus accumbens of mice
Chronic methamphetamine regulates the expression of MicroRNAs and putative target genes in the nucleus accumbens of mice
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DOI：
10.1002/jnr.23605
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发表时间：
2015-10
影响因子：
4.2
通讯作者：
Li Zhu;Jie Zhu;Yufeng Liu;Yanjiong Chen;Yanlin Li;Sisi Chen;Tao Li;Yonghui Dang;Teng Chen
中科院分区：
文献类型：
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作者：
Li Zhu;Jie Zhu;Yufeng Liu;Yanjiong Chen;Yanlin Li;Sisi Chen;Tao Li;Yonghui Dang;Teng Chen
microRNAs（miRNAs）是基因表达的调节因子，在不同的细胞过程中发挥关键的调控作用。甲基苯丙胺（METH）通过影响复杂的基因调控机制诱导边缘系统的各种异常变化，但在METH暴露的影响中miRNA的参与仍不清楚。这项研究确定了METH-反应性miRNA及其在小鼠延髓核（NAc）中的潜在作用。使用miRNA测序，我们检测了盐水和METH处理小鼠NAc中miRNA的表达，并鉴定了45种已知的METH应答性miRNA。此外，我们还鉴定了两种新的METH反应性miRNA候选物（novel-m002 C和novel-m009 C）。我们的靶点预测分析表明，已知的METH调节的miRNA可能靶向参与细胞自噬、细胞代谢和免疫反应的基因，而新的METH调节的miRNA候选物可能靶向与药物成瘾相关的基因。我们还将METH调节的miRNA的预测靶点与NAc信使RNA表达谱进行了匹配，揭示了8个推定的METH调节靶基因（Arc，Capn 9，Gbp 5，Lefty 1，Patl 2，Pde 4c，Strc和Vmn 1 r58）。因此，METH触发NAc miRNA表达的改变，这可能有助于METH诱导的神经元自噬、代谢和免疫反应的变化。推定的靶基因的差异表达表明它们参与了暴露于METH后的反应。© 2015威利期刊公司.
MicroRNAs (miRNAs) are modulators of gene expression that play key regulatory roles in distinct cellular processes. Methamphetamine (METH) induces various aberrant changes in the limbic system by affecting a complex gene regulatory mechanism, yet the involvement of miRNAs in the effects of METH exposure remains unclear. This study identifies METH‐responsive miRNAs and their potential effects in the nucleus accumbens (NAc) of mice. Using miRNA sequencing, we examined the expression of miRNAs in the NAc of saline‐ and METH‐treated mice and identified 45 known miRNAs to be METH responsive. Additionally, we identified two novel miRNA candidates that were METH responsive (novel‐m002C and novel‐m009C). Our target prediction analysis suggested that the known METH‐regulated miRNAs might target genes that are involved in cellular autophagy, cellular metabolism, and immune responses and that the novel METH‐regulated miRNA candidates might target genes that are related to drug addiction. We also matched the predicted targets of METH‐regulated miRNAs with the NAc messenger RNA expression profile, revealing eight putative METH‐regulated target genes (Arc, Capn9, Gbp5, Lefty1, Patl2, Pde4c, Strc, and Vmn1r58). Thus, METH triggers an alteration in NAc miRNA expression, which could contribute to METH‐induced changes in neuron autophagy, metabolism, and immune responses. The differential expression of putative target genes suggests their involvement following exposure to METH. © 2015 Wiley Periodicals, Inc.