CDT1 IS AN ESSENTIAL TARGET OF THE CDC10/SCT1 TRANSCRIPTION FACTOR - REQUIREMENT FOR DNA-REPLICATION AND INHIBITION OF MITOSIS
CDT1 IS AN ESSENTIAL TARGET OF THE CDC10/SCT1 TRANSCRIPTION FACTOR - REQUIREMENT FOR DNA-REPLICATION AND INHIBITION OF MITOSIS
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DOI：
10.1002/j.1460-2075.1994.tb06277.x
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发表时间：
1994-01-15
期刊：
影响因子：
11.4
通讯作者：
BEACH, D
中科院分区：
文献类型：
--
作者：
HOFMANN, JFX;BEACH, D
我们已经使用了免疫沉淀- PCR循环，以物理方式分离基因组DNA序列，这些DNA序列与裂殖酵母cdc 10基因产物结合，试图鉴定新的靶基因。一个必需基因cdt 1已被分离出来，其表达受cdc 10依赖性方式的细胞周期调控。cdt 1启动子含有序列特异性DNA结合因子的识别位点。cdc 10基因产物是该因子的组成部分。cdt 1的异位表达可以在半允许温度下补充cdc 10的温度敏感突变。携带cdt 1的无效等位基因的细胞在DNA复制中有缺陷，但启动有丝分裂事件，这表明cdt 1是S期和有丝分裂的正常依赖关系所必需的。
We have used an immunoprecipitation - PCR cycle to isolate physically genomic DNA sequences that are bound by the fission yeast cdc10 gene product in an attempt to identify novel target genes. An essential gene, cdt1, has been isolated whose expression is cell cycle regulated in a cdc10 dependent manner. The cdt1 promoter contains a recognition site for a sequence specific DNA binding factor. The cdc10 gene product is a component of this factor. Ectopic expression of cdt1 can complement a temperature sensitive mutation of cdc10 at semipermissive temperature. Cells carrying a null allele of cdt1 are defective in DNA replication but initiate mitotic events, suggesting that cdt1 is essential for the normal dependency relationship of S-phase and mitosis.