GPA1, A HAPLOID-SPECIFIC ESSENTIAL GENE, ENCODES A YEAST HOMOLOG OF MAMMALIAN G-PROTEIN WHICH MAY BE INVOLVED IN MATING FACTOR SIGNAL TRANSDUCTION
GPA1, A HAPLOID-SPECIFIC ESSENTIAL GENE, ENCODES A YEAST HOMOLOG OF MAMMALIAN G-PROTEIN WHICH MAY BE INVOLVED IN MATING FACTOR SIGNAL TRANSDUCTION
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DOI：
10.1016/0092-8674(87)90167-x
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发表时间：
1987-09-25
期刊：
影响因子：
64.5
通讯作者：
MATSUMOTO, K
中科院分区：
文献类型：
--
作者：
MIYAJIMA, I;NAKAFUKU, M;MATSUMOTO, K
酿酒酵母的GPA 1蛋白与α-葡萄糖苷酶同源。哺乳动物G蛋白亚基。在二倍体细胞中未检测到GPA 1的转录。GPA 1的破坏导致单倍体特异性致死表型，表明GPA 1是细胞生长的单倍体特异性必需基因。通过半乳糖诱导型GAL 1启动子调控GPA 1的表达，发现GPA 1功能的丧失导致细胞周期停滞在G1期晚期。抑制gpa 1：：HIS 3突变致死性的突变体显示无菌表型，其不是细胞类型特异性的。这些结果表明，GPA 1蛋白可能控制信号交配因子介导的细胞周期阻滞。
GPA1 protein of Saccharomyces cerevisiae is homologous to the .alpha. subunit of mammalian G protein. GPA1 transcript was found in haploid cells but was not detected in diploid cells. Disruption of GPA1 resulted in a haploid-specific lethal phenotype, indicating that GPA1 is a haploid-specific essential gene for cell growth. Upon regulation of expression of GPA1 by the galactose-inducible GAL1 promoter, the loss of GPA1 function was found to lead to cell-cycle arrest at the late G1 phase. Mutants that suppress the lethality of the gpa1::HIS3 mutation showed a sterile phenotype that was not cell-type-specific. These results suggest that GPA1 protein may control the signal for mating-factor-mediated cell-cycle arrest.