Asymmetric uptake of sepiapterin and 7,8-dihydrobiopterin as a gateway of the salvage pathway of tetrahydrobiopterin biosynthesis from the lumenal surface of rat endothelial cells
Asymmetric uptake of sepiapterin and 7,8-dihydrobiopterin as a gateway of the salvage pathway of tetrahydrobiopterin biosynthesis from the lumenal surface of rat endothelial cells
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DOI：
10.1016/j.ymgme.2011.06.003
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发表时间：
2011-11-01
影响因子：
3.8
通讯作者：
Hasegawa, Hiroyuki
中科院分区：
文献类型：
--
作者：
Ohashi, Akiko;Mamada, Kaori;Hasegawa, Hiroyuki
将大鼠主动脉内皮细胞培养在多孔膜上，形成单层膜。它们通过摄取Sepiapterin有效地积累了四氢生物蝶呤(BH4)，但通过摄取二氢生物蝶呤只能适度地积累BH4。内皮细胞片优先从根尖侧摄取蝶呤类药物。相应地，在细胞板的顶面可见密集的ent2样免疫反应积聚。这一发现表明，血管内皮细胞直接从血流而不是从腔组织接收BH4前体。(C)2011 Elsevier Inc.保留所有权利。
Rat aortic endothelial cells were cultured on a porous membrane to form a monolayer sheet. They efficiently accumulated tetrahydrobiopterin (BH4) by uptake of sepiapterin but did so only moderately by uptake of dihydrobiopterin. The endothelial cell sheet preferentially took up the pterins from the apical side. Accordingly, a dense accumulation of ENT2-like immunoreactivity was visualized on the apical surface of the cell sheet. The findings suggest that vascular endothelial cells receive BH4 precursors directly from the blood stream rather than from ablumenal tissues. (C) 2011 Elsevier Inc. All rights reserved.