Boron Deficiency Effects on Sugar, Ionome, and Phytohormone Profiles of Vascular and Non-Vascular Leaf Tissues of Common Plantain (Plantago major L.)
Boron Deficiency Effects on Sugar, Ionome, and Phytohormone Profiles of Vascular and Non-Vascular Leaf Tissues of Common Plantain (Plantago major L.)
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DOI：
10.3390/ijms20163882
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发表时间：
2019-08
影响因子：
5.6
通讯作者：
Benjamin Pommerrenig;K. Eggert;G. Bienert
中科院分区：
文献类型：
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作者：
Benjamin Pommerrenig;K. Eggert;G. Bienert
维管组织基本上调节整个植物体的水分、养分、光合作用和植物激素物流。硼（B）对许多双子叶植物维管组织的发育至关重要，而B缺乏尤其影响韧皮部和木质部导管的完整性，并因此影响长距离运输的功能。我们假设植物B营养状态的变化引起了脉管系统和叶肉的不同反应。然而，直接分析的血管系统在B缺乏的反应是缺乏的，由于该组织的实验不可及性。在这里，我们产生了生化和生理的理解B缺乏反应反应，在共同大蕉（车前草L.），从中可以提取纯净和完整的维管束。低土壤B浓度影响各种植物激素，糖和宏量和微量营养素在组织特异性的方式的数量分布格局。在低B供应下，维管蔗糖水平下降，韧皮部的蔗糖负荷减少。植物激素选择性地响应B剥夺。虽然脱落酸和水杨酸的浓度下降，在低B供应，细胞分裂素和油菜素类固醇增加，分别在脉管系统和叶肉。我们的研究结果强调了以组织而不是器官特异性方式分析营养素缺乏反应的生物学必要性。
Vascular tissues essentially regulate water, nutrient, photo-assimilate, and phytohormone logistics throughout the plant body. Boron (B) is crucial for the development of the vascular tissue in many dicotyledonous plant taxa and B deficiency particularly affects the integrity of phloem and xylem vessels, and, therefore, functionality of long-distance transport. We hypothesize that changes in the plants’ B nutritional status evoke differential responses of the vasculature and the mesophyll. However, direct analyses of the vasculature in response to B deficiency are lacking, due to the experimental inaccessibility of this tissue. Here, we generated biochemical and physiological understanding of B deficiency response reactions in common plantain (Plantago major L.), from which pure and intact vascular bundles can be extracted. Low soil B concentrations affected quantitative distribution patterns of various phytohormones, sugars and macro-, and micronutrients in a tissue-specific manner. Vascular sucrose levels dropped, and sucrose loading into the phloem was reduced under low B supply. Phytohormones responded selectively to B deprivation. While concentrations of abscisic acid and salicylic acid decreased at low B supply, cytokinins and brassinosteroids increased in the vasculature and the mesophyll, respectively. Our results highlight the biological necessity to analyze nutrient deficiency responses in a tissue- rather organ-specific manner.