Intraspecific variation in realized dispersal probability and host quality shape nectar microbiomes
Intraspecific variation in realized dispersal probability and host quality shape nectar microbiomes
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DOI：
10.1111/nph.19195
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发表时间：
2023-11-01
期刊：
影响因子：
9.4
通讯作者：
Vannette，Rachel L.
中科院分区：
文献类型：
--
作者：
Francis，Jacob S.;Mueller，Tobias G.;Vannette，Rachel L.
附生微生物经常影响植物表型和适合度，但其影响取决于微生物丰度和群落组成。植物性状过滤和确定性传播介导的过程可以影响微生物组组装，但它们对微生物组可预测变化的相对贡献知之甚少。我们比较了宿主植物过滤和传播对花蜜微生物组存在、丰度和组成的影响。我们将具有代表性的细菌和酵母接种到四个表型不同的柳叶菜品种的30株植物中。我们比较了接种群落和开放访问的同一植物的一个子集的花的生长情况。当我们用合成群落接种花时，有明显的宿主选择证据。然而，当接种时具有最高微生物密度的植物在公开访问时没有最高的微生物密度。相反，植物在细菌的存在下，这是与花粉接收和花卉性状，这表明确定性dispersional.These研究结果表明，主机过滤的作用可以驱动植物微生物组组装在组织中的物种池是大的，分散是高的。然而，当微生物依赖于动物媒介、传播率低或到达顺序重要时，微生物传播到宿主的确定性差异可能同样重要或更重要。
Epiphytic microbes frequently affect plant phenotype and fitness, but their effects depend on microbe abundance and community composition. Filtering by plant traits and deterministic dispersal‐mediated processes can affect microbiome assembly, yet their relative contribution to predictable variation in microbiome is poorly understood.We compared the effects of host‐plant filtering and dispersal on nectar microbiome presence, abundance, and composition. We inoculated representative bacteria and yeast into 30 plants across four phenotypically distinct cultivars ofEpilobium canum. We compared the growth of inoculated communities to openly visited flowers from a subset of the same plants.There was clear evidence of host selection when we inoculated flowers with synthetic communities. However, plants with the highest microbial densities when inoculated did not have the highest microbial densities when openly visited. Instead, plants predictably varied in the presence of bacteria, which was correlated with pollen receipt and floral traits, suggesting a role for deterministic dispersal.These findings suggest that host filtering could drive plant microbiome assembly in tissues where species pools are large and dispersal is high. However, deterministic differences in microbial dispersal to hosts may be equally or more important when microbes rely on an animal vector, dispersal is low, or arrival order is important.