H CHAIN SUBGROUPS OF MYELOMA PROTEINS AND NORMAL 7S GAMMA-GLOBULIN.
H CHAIN SUBGROUPS OF MYELOMA PROTEINS AND NORMAL 7S GAMMA-GLOBULIN.
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DOI：
10.1084/jem.120.2.253
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发表时间：
1964-08-01
期刊：
影响因子：
--
通讯作者：
KUNKEL HG
中科院分区：
文献类型：
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作者：
GREY HM;KUNKEL HG
通过使用各种针对分离的骨髓瘤蛋白的抗血清，可以很容易地区分7Sγ球蛋白类型的四个亚群。第一个亚群是Vi亚群，由10个骨髓瘤蛋白组成。第二组，We组，包含了大多数骨髓瘤蛋白。第三个是GE亚组，包括50种骨髓瘤蛋白中的3种。第四种定义仍然不明确，而且看起来是异质的。在所有研究的个体血清中，在Fr IIγ球蛋白和正常的γ球蛋白中都发现了Vi和Ge亚群的对应物。尽管不同的抗血清涉及不同的决定因素，但两组独特的抗原性都定位在H链上。在某些兔抗血清中，完整的二硫键起着重要的作用。除了特殊的抗原特性外，GE亚群在每一种情况下都表现出对木瓜酶产生的F片段的快速迁移率，这是其他骨髓瘤蛋白所没有的。
Through the use of a variety of antisera to isolated myeloma proteins, four subgroups of 7S γ-globulin type proteins were readily distinguished. The first, the Vi subgroup, consisted of ten of 64 myeloma proteins studied. The second, the We group, contained the majority of myeloma proteins. The third, the Ge subgroup, included three of 50 myeloma proteins. The fourth remains ill-defined and appears heterogeneous. Counterparts for both the Vi and the Ge subgroup, were found in the Fr II γ-globulin and in the normal γ-globulin of all of a large number of individual sera studied. The unique antigenic character of both groups was localized to the H chains, although different determinants were involved for different antisera. An essential role of intact disulfide bonds was apparent with certain rabbit antisera. In addition to the special antigenic characteristics, the Ge subgroup showed in each instance a fast mobility for the F fragments produced by papain which was not found for other myeloma proteins.