Production of metabolites with antioxidant activity by Botryosphaeria dothidea in submerged fermentation
Production of metabolites with antioxidant activity by Botryosphaeria dothidea in submerged fermentation
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DOI：
10.1007/s00449-019-02200-y
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发表时间：
2020-01-01
影响因子：
3.8
通讯作者：
Terra, Lisiane de Marsillac
中科院分区：
文献类型：
--
作者：
Druzian, Susanne Pedroso;Pinheiro, Luiza Negri;Terra, Lisiane de Marsillac
抗氧化剂能够抑制自由基，在人类饮食、食品保护、抗衰老化妆品等方面发挥着重要作用。真菌在产生具有生物活性的代谢物（例如抗氧化剂）方面受到重视。这项研究的主要目的是从巴西潘帕和大西洋森林生物群落的资源中选择一种能够通过深层发酵生产抗氧化剂的真菌。测定了化合物对自由基 2.2-二苯基-1-三硝基苯肼 (DPPH)、超氧化物和羟基的清除活性。 Botryosphaeria dothidea表现出较高的抗氧化剂生产潜力，其上清液对DPPH、超氧化物和羟基自由基的抑制百分比分别为94.47、94.87和89.78%，IC50为0.206 mg/mL。对肉汤中存在的挥发性化合物的鉴定证实了六氢吡咯嗪-3-酮的存在； 1，2-苯二甲酸双(2-甲基丙基)酯和3，6-双(2-甲基丙基)-2，5-哌嗪二酮。根据这项研究，Botryosphaeria dothidea 具有生产具有抗氧化活性的化合物的巨大潜力。
Antioxidants are able of inhibiting free radicals and play an important role in human diet, protection of foods, anti-aging cosmetics, among others. Fungi have been highlighted in production of metabolites with biological activity, such as antioxidant. The main objective of this study was to select a fungus from resources of Brazilians Pampa and Atlantic Forest biomes with hability for production of antioxidants by submerged fermentation. The scavenging activity of the compounds was determined against radical 2.2-diphenyl-1-picrylhydrazyl (DPPH), superoxide and hydroxyl. Botryosphaeria dothidea exhibited higher potential for the production of antioxidants, and its supernatant showed percentage inhibition values of 94.47, 94.87 and 89.78% against DPPH, superoxide and hydroxyl radicals and IC50 of 0.206 mg/mL. The identification of the volatile compounds present in the broth verified the presence of the hexahydropyrrolizin-3-one; 1.2-benzenedicarboxylic acid bis(2-methylpropyl) ester and 3.6-bis(2-methylpropyl)-2.5-piperazinedione. According to this study, Botryosphaeria dothidea presents great potential to produce compounds with antioxidant activity.