Transforming growth factor-β2 levels in aqueous humor of glaucomatous eyes
Transforming growth factor-β2 levels in aqueous humor of glaucomatous eyes
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DOI：
10.1007/s004170000241
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发表时间：
2001-02-01
影响因子：
2.7
通讯作者：
Honda, Y
中科院分区：
文献类型：
--
作者：
Inatani, M;Tanihara, H;Honda, Y
目的：确定青光眼患者的临床特征是否与房水中转化生长因子-β（2）（TGF-β（2））水平升高相关。方法：收集91例青光眼患者的房水标本。其中原发性开角型青光眼（POAG）40眼，（假性）剥脱综合征（EXS）18眼，原发性闭角型青光眼（PACG）26眼，葡萄膜炎相关继发性青光眼（SG）7眼。用特异性捕获ELISA评估房水中的TGF-β（2）。结果如下：平均浓度（+/-标准误差）成熟POAG眼房水中的（生物活性）TGF-β 2为293.6 +/- 33.6 pg/ml，显著高于PACG、EXS和SG眼：分别为147.5 ± 28.1、135.8 ± 30.2和41.0 ± 10.7 pg/ml（P=0.0006、P=0.0010和P=0.0003;方差分析）。POAG眼房水中总TGF-β 2的平均浓度（rt标准误）为1647.6 +/- 124.5 pg/ml，与PACG、EXS和SG眼无显著差异：分别为1482.9 +/- 148.2、1442.7 +/- 187.8和1929.0 +/- 367.6 pg/ml。使用逻辑回归的多变量分析显示，成熟TGF-β 2浓度与白内障手术史（P=0.0225）和碳酸酐酶抑制剂的使用（P=0.0133）之间存在显著相关性。结论：我们的研究结果表明，TGF-β（2）水平的升高可能在POAG的发病机制中起重要作用。
Purpose: To determine whether clinical characteristics are correlated with increased levels of transforming growth factor-beta (2) (TGF-beta (2)) in aqueous humor in glaucomatous eyes. Methods: Aqueous humor samples were collected from 91 glaucomatous eyes. Included were samples from primary open-angle glaucoma (POAG) in 40 eyes, (pseudo)exfoliation syndrome (EXS) in 18 eyes, primary angle-closure glaucoma (PACG) in 26 eyes and uveitis-related secondary glaucoma (SG) in 7 eyes. TGF-beta (2) in aqueous humor was assessed with a specific-capture ELISA. Results: The mean concentration (+/- standard error) of mature (biologically active) TGF-beta (2) in the aqueous humor of eyes with POAG was 293.6 +/- 33.6 pg/ml, significantly higher than that in eyes with PACG, EXS and SG: 147.5 +/- 28.1, 135.8 +/- 30.2 and 41.0 +/- 10.7 pg/ml, respectively (P=0.0006, P=0.0010 and P=0.0003; analysis of variance). The mean concentration (rt standard error) Of total TGF-beta (2) in the aqueous humor of eyes with POAG was 1647.6 +/- 124.5 pg/ml, not significantly different from that in eyes with PACG, EXS and SG: 1482.9 +/- 148.2, 1442.7 +/- 187.8 and 1929.0 +/- 367.6 pg/ml, respectively. A multivariate analysis using logistic regression showed significant correlations between mature TGF-beta (2) concentration and history of cataract surgery (P=0.0225) and the use of carbonic anhydrase inhibitors (P=0.0133). Conclusions: Our results indicate that increased levels of TGF-beta (2) may play an important role in the pathogenesis of POAG.