AN ENZYME-LINKED IMMUNOSORBENT-ASSAY USING CANINE CORONAVIRUS-INFECTED CRFK CELLS AS ANTIGEN FOR DETECTION OF ANTI-CORONAVIRUS ANTIBODY IN CAT
AN ENZYME-LINKED IMMUNOSORBENT-ASSAY USING CANINE CORONAVIRUS-INFECTED CRFK CELLS AS ANTIGEN FOR DETECTION OF ANTI-CORONAVIRUS ANTIBODY IN CAT
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DOI：
10.1016/0147-9571(89)90062-3
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发表时间：
1989-01-01
影响因子：
2
通讯作者：
FURUKAWA, H
中科院分区：
文献类型：
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作者：
MOCHIZUKI, M;FURUKAWA, H
由于犬冠状病毒（CCV）在细胞内的生长效率比猫冠状病毒高，并且它们的抗原性相互相关，因此开发了一种使用CCV感染的猫肾（CRFK）细胞作为底物抗原的酶联免疫吸附测定（ELISA）来检测猫的抗冠状病毒抗体。为了产生冠状病毒特异性 ELISA 抗体活性，将样品与 CCV 感染细胞平行应用于模拟感染的正常 CRFK 细胞，然后从病毒感染细胞抗原给出的光密度值中减去模拟感染细胞抗原给出的光密度值。根据从实验性感染 CCV 的猫和自发性猫传染性腹膜炎 (FIP) 病例的血清中获得的 ELISA 抗体滴度，发现本研究中描述的 ELISA 可作为一种简单易行的血清学测试，能够与同源 FIP 病毒的间接免疫荧光测定一样有效地检测抗冠状病毒抗体。
From the reasons that canine coronavirus (CCV) grows more efficiently than feline coronavirus in a cell and they are mutually related in their antigenicities, an enzyme-linked immunosorbent assay (ELISA) using CCV-infected feline kidney (CRFK) cells as substrate antigens was developed for detection of anti-coronavirus antibodies in cats. It was indispensable for generating coronavirus-specific ELISA antibody activities that the sample was applied to the mock-infected, normal CRFK cells in parallel with the CCV-infected cells and then the optical density values given by the mock-infected cell antigen were subtracted from those given by the virus-infected cell antigen. On the basis of ELISA antibody titers obtained in sera from the cats experimentally infected with CCV and from the spontaneous feline infectious peritonitis (FIP) cases, the ELISA described in the present study was found to be applicable as a simple and easy serologic test which was able to detect anti-coronavirus antibodies as efficiently as the indirect immunofluorescence assay with homologous FIP virus.