Cdc13 at a crossroads of telomerase action.
Cdc13 at a crossroads of telomerase action.
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DOI：
10.3389/fonc.2013.00039
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发表时间：
2013
影响因子：
4.7
通讯作者：
Géli V
中科院分区：
文献类型：
--
作者：
Churikov D;Corda Y;Luciano P;Géli V
端粒酶延长端粒涉及一系列连续步骤，这些步骤必须高度协调，以确保端粒维持在适当的长度。端粒酶被递送到端粒末端，在那里它与单链 DNA 末端作为引物接合，将其延长，并通过可能与互补 C 链的合成耦合的机制从端粒解离。在酿酒酵母中，端粒 G 突出端结合的 Cdc13 充当招募多个因子的平台，这些因子协调这些步骤之间的及时转换。在这篇综述中，我们重点关注端粒酶招募的一些未解决的方面，以及端粒酶招募到染色体末端后调节端粒伸长的机制。我们还强调了 Cdc13 的关键监管修改，这些修改促进了端粒延长步骤之间的转换。
Telomere elongation by telomerase involves sequential steps that must be highly coordinated to ensure the maintenance of telomeres at a proper length. Telomerase is delivered to telomere ends, where it engages single-strand DNA end as a primer, elongates it, and dissociates from the telomeres via mechanism that is likely coupled to the synthesis of the complementary C-strand. In Saccharomyces cerevisiae, the telomeric G-overhang bound Cdc13 acts as a platform for the recruitment of several factors that orchestrate timely transitions between these steps. In this review, we focus on some unresolved aspects of telomerase recruitment and on the mechanisms that regulate telomere elongation by telomerase after its recruitment to chromosome ends. We also highlight the key regulatory modifications of Cdc13 that promote transitions between the steps of telomere elongation.