Dietary docosahexaenoic acid reverses nonalcoholic steatohepatitis and fibrosis caused by conjugated linoleic acid supplementation in mice
Dietary docosahexaenoic acid reverses nonalcoholic steatohepatitis and fibrosis caused by conjugated linoleic acid supplementation in mice
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膳食二十二碳六烯酸可逆转补充共轭亚油酸引起的小鼠非酒精性脂肪性肝炎和纤维化
DOI：
10.1016/j.jff.2015.11.028
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发表时间：
2016-01-01
影响因子：
5.6
通讯作者：
Kelley, Darshan S.
中科院分区：
文献类型：
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作者：
Adkins, Yuriko;Fedor, Dawn M.;Kelley, Darshan S.
研究表明，二十二碳六烯酸 (DHA) 可以预防非酒精性脂肪肝 (NAFLD) 和由共轭亚油酸 (CLA)（一种反式脂肪酸 (TFA)）引起的胰岛素抵抗 (IR)。在这里，我们评估了 DHA 是否会逆转现有的 CLA 诱导的小鼠 NAFLD 和 IR。 DHA 对现有 NAFLD 的特异性作用包括显着（P < 0.005）降低肝脏重量和三酰甘油含量以及参与脂肪酸合成的基因表达，增强参与脂肪酸氧化的基因表达，并增加血清脂联素水平。此外，免疫组织化学显示肝脏 CD163（炎症）和平滑肌 a-肌动蛋白（纤维化）的表达较低。与 CLA 饮食相比，喂食 DHA 和对照饮食的小鼠血清胰岛素和 ALT 活性显着降低（P < 0.05），但只有 DHA 参与纤维化的基因表达降低（P = 0.05）。补充 DHA 4 周可逆转由 CLA 引起的现有肝脏脂肪变性、炎症和纤维化。由爱思唯尔有限公司出版
It has been shown that docosahexaenoic acid (DHA) prevents nonalcoholic fatty liver disease (NAFLD) and insulin resistance (IR) caused by conjugated linoleic acid (CLA), a trans fatty acid (TFA). Here, we evaluated whether DHA will reverse existing CLA-induced NAFLD and IR in mice. DHA-specific effects on existing NAFLD involved significant (P < 0.005) lowering of hepatic weight and triacylglycerol content and expression of genes involved in fatty acid synthesis, enhancing expression of genes involved in fatty acid oxidation, and increasing serum adiponectin levels. Also, immunohistochemistry showed lower expression of hepatic CD163 (inflammation) and smooth muscle a-actin (fibrosis). Compared to the CLA diet, mice fed DHA and control diets had significantly (P < 0.05) lower serum insulin and ALT activity, but only DHA had lower (P = 0.05) expression of genes involved in fibrosis. DHA supplementation for 4 weeks reversed already existing hepatic steatosis, inflammation, and fibrosis caused by CLA. Published by Elsevier Ltd.