Protection against a European H1N2 swine influenza virus in pigs previously infected with H1N1 and/or H3N2 subtypes
Protection against a European H1N2 swine influenza virus in pigs previously infected with H1N1 and/or H3N2 subtypes
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DOI：
10.1016/s0264-410x(02)00688-6
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发表时间：
2003-03-28
期刊：
影响因子：
5.5
通讯作者：
Pensaert, M
中科院分区：
文献类型：
--
作者：
Van Reeth, K;Gregory, V;Pensaert, M
一种新的猪流感病毒H1 N2与H1N1和H3 N2病毒一起在欧洲猪群中传播。本研究检测感染后对H1N1和/或H3 N2病毒的免疫是否提供了对H1 N2感染的交叉保护。猪（n = 51）鼻内接种Sw/Belgium/1/98（H1 N1）或Sw/弗兰德斯/1/98（H3 N2），或每隔5周接种两种病毒。对照组不接种或接种Sw/Gent/7625/99（H1 N2）。4周后，所有猪均经鼻内和肠道内接种高剂量H1 N2病毒。攻毒对照猪表现出典型的流感症状，在6或7天内，肺部和鼻腔病毒排泄物中均出现高H1 N2病毒滴度。H1 N2免疫猪表现出完全的临床和病毒学保护。仅对H1N1或H3 N2免疫的猪不能防止疾病和病毒在肺中复制，但病毒排泄缩短了2天。相比之下，对H1N1和H3 N2免疫的猪没有表现出疾病，H1 N2病毒复制要么检测不到，要么明显减少。血凝抑制（HI）和病毒中和（VN）试验表明，针对H1 N2的交叉保护可能不是由针对血凝素（HA）的抗体介导的。抑制H1 N2的神经氨酸酶（NA）的抗体在H3 N2单独免疫猪中处于最低水平，但它们在（H1N1 + H3 N2）免疫猪中始终存在。针对H1N1、H3 N2和H1 N2病毒中相对保守的内部蛋白的免疫应答可能在针对H1 N2的保护中起重要作用。考虑到这里使用的严重攻击模型，在自然感染条件下，针对H1 N2的交叉保护可能更明显。(C)2002爱思唯尔科技有限公司版权所有。
A novel swine influenza virus, H1N2, circulates in European swine populations together with H1N1 and H3N2 viruses. This study examines whether post-infection immunity to H1N1 and/or H3N2 viruses provides cross-protection against H1N2 infection. Pigs (n = 51) were inoculated intranasally with either Sw/Belgium/1/98 (H1N1) or Sw/Flanders/1/98 (H3N2), or with both viruses at a 5-week interval. Control groups were left uninoculated or inoculated with Sw/Gent/7625/99 (H1N2). Four weeks later, all the pigs were challenged intranasally and intratracheally with a high H1N2 virus dose. The challenge control pigs showed typical influenza symptoms, and all had high H1N2 virus titres in the lungs and nasal virus excretion during 6 or 7 days. The H1N2-immune pigs showed total clinical and virological protection. Pigs immune against H1N1 or H3N2 only were not protected against disease and virus replication in the lungs, but virus excretion was 2 days shorter. By contrast, pigs immune against both H1N1 and H3N2 did not show disease and H1N2 virus replication was either undetectable or markedly reduced. Haemagglutination inhibition (HI) and virus neutralisation (VN) tests indicated that cross-protection against H1N2 was probably not mediated by antibodies against the haemagglutinin (HA). Antibodies inhibiting the neuraminidase (NA) of H1N2 were at minimal levels in H3N2 only-immune pigs, but they were consistently found in (H1N1 + H3N2)-immune pigs. The immune response against the internal proteins, which are relatively conserved in H1N1, H3N2 and H1N2 viruses, may play a significant role in protection against H1N2. Given the severe challenge model used here, cross-protection against H1N2 could be more pronounced under natural conditions of infection. (C) 2002 Elsevier Science Ltd. All rights reserved.