A tessellated lymphoid network provides whole-body T cell surveillance in zebrafish.
A tessellated lymphoid network provides whole-body T cell surveillance in zebrafish.
复制标题
棋盘状淋巴网络可对斑马鱼进行全身 T 细胞监测。
DOI：
10.1101/2023.01.17.524414
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Huttenlocher,Anna
中科院分区：
文献类型：
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作者：
Robertson,TannerF;Hou,Yiran;Shen,Simone;Rindy,Julie;Sauer,John-Demian;Dinh,HuyQ;Huttenlocher,Anna
稳态运输到淋巴结允许T细胞有效地调查宿主的同源抗原。非哺乳类有颚脊椎动物缺乏淋巴结，但维持不同的T细胞池。在这里，我们利用透明斑马鱼的体内成像来研究T细胞如何组织和调查没有淋巴结的动物中的抗原。我们发现斑马鱼中的幼稚样T细胞组织成一个以前未描述的全身淋巴网络，支持流式迁移和通过宿主的协调运输。该网络具有哺乳动物淋巴结的细胞标志，包括幼稚T细胞和表达CCR7配体的非造血细胞，并促进快速集体迁移。在感染期间，T细胞转变为支持抗原呈递细胞相互作用和随后激活的随机游走。我们的研究结果表明，T细胞可以在集体迁移和个体随机游走之间切换，以优先考虑大规模运输或原位抗原搜索。因此，这种淋巴网络在没有淋巴结系统的情况下促进全身T细胞运输和抗原监视。
Homeostatic trafficking to lymph nodes allows T cells to efficiently survey the host for cognate antigen. Nonmammalian jawed vertebrates lack lymph nodes but maintain diverse T cell pools. Here, we exploit in vivo imaging of transparent zebrafish to investigate how T cells organize and survey for antigen in an animal devoid of lymph nodes. We find that naïve-like T cells in zebrafish organize into a previously undescribed whole-body lymphoid network that supports streaming migration and coordinated trafficking through the host. This network has the cellular hallmarks of a mammalian lymph node, including naïve T cells and CCR7-ligand expressing nonhematopoietic cells, and facilitates rapid collective migration. During infection, T cells transition to a random walk that supports antigen-presenting cell interactions and subsequent activation. Our results reveal that T cells can toggle between collective migration and individual random walks to prioritize either large-scale trafficking or antigen search in situ. This lymphoid network thus facilitates whole-body T cell trafficking and antigen surveillance in the absence of a lymph node system.