Immunologic properties of conjugates of ragweed antigen E with various alkoxypolyethylene glycols.
Immunologic properties of conjugates of ragweed antigen E with various alkoxypolyethylene glycols.
复制标题
豚草抗原 E 与各种烷氧基聚乙二醇缀合物的免疫学特性。
DOI：
10.1159/000232852
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
C. Weiner
中科院分区：
文献类型：
--
作者：
T. P. King;C. Weiner
来自豚草花粉的抗原 E 已通过将其 18 个ε-氨基中的约 8 个与各种烷氧基聚乙二醇 (ROPEG) 偶联进行修饰。这些二醇包括 2,000 和 5,000 道尔顿的甲氧基-PEG、1,200 道尔顿的正月桂酰氧基-PEG (BRIJ-35) 和 3,300 道尔顿的对异辛基苯氧基-PEG (Triton X-705)。在小鼠中测试了这些缀合物的免疫原性和免疫抑制活性。尽管 BRIJ-35 缀合物仅显示出轻微降低的免疫原性，但它们显示出抗原 E 特异性 IgE 和 IgG 抗体反应的免疫原性降低。缀合物分子的蛋白质部分似乎含有与天然抗原E相同的抗原决定簇。缀合物分子的烷氧基聚乙二醇氧基部分被发现具有弱免疫原性，因为用此类缀合物免疫的小鼠表现出短暂的弱IgE抗体应答。所有缀合物保留了抗原E的免疫抑制特性，因为用高剂量的抗原E或缀合物对抗原E致敏的小鼠进行皮下治疗导致其特异性IgE和IgG抗体水平受到抑制。
Antigen E from ragweed pollen has been modified by coupling about 8 of its 18 epsilon-amino groups with various alkoxypolyethylene glycols (ROPEG). These glycols include methoxy-PEGs of 2,000 and 5,000 daltons, n-laruyloxy-PEG of 1,200 daltons (BRIJ-35), and p-isooctylpohenoxy-PEG of 3,300 daltons (Triton X-705). The immunogenic and immunosuppressive activities of these conjugates were tested in mice. They showed reduced immunogenicity for antigen E-specific IgE and IgG antibody responses although the BRIJ-35 conjugate showed only slightly decreased immunogenicity. The protein portion of the conjugate molecules appeared to contain the same antigenic determinants as in native antigen E. The alkoxypolyethyleneglycoxy portion of the conjugate molecules was found to be weakly immunogenic, since mice which had been immunized with such conjugates showed a transient weak IgE antibody response. All conjugates retained the immunosuppressive property of antigen E since the subcutaneous treatment of antigen E-sensitized mice with high doses of antigen E or of conjugate led to suppression of their specific IgE and IgG antibody levels.