THE CYTOSKELETON OF SPREADING DICTYOSTELIUM AMEBAS
THE CYTOSKELETON OF SPREADING DICTYOSTELIUM AMEBAS
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DOI：
10.1007/bf01276319
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发表时间：
1987-01-01
期刊：
影响因子：
2.9
通讯作者：
SMALL, JV
中科院分区：
文献类型：
--
作者：
RUBINO, S;SMALL, JV
在用戊二醛和 Triton-X-100 的混合物制备的细胞中，通过光学和电子显微镜观察盘基网柄菌阿米巴的细胞结构元素。负染色后，阿米巴扩散的外围区域显示出复杂的肌动蛋白丝网状结构，其中大部分长度小于 0.25 微米。多个分支点、端对侧邻接和交叉是肌动蛋白网络的特征。从细胞外围延伸的丝状伪足由肌动蛋白丝束组成，这些肌动蛋白丝渗透到展开的片层中的肌动蛋白网状结构中并与其融合。从核相关体发出的微管不同程度地渗透到肌动蛋白网中，有时延伸到靠近细胞外围。按此处所述制备的盘基网柄菌细胞骨架应该对运动机制的进一步研究有用。
The cytoarchitectural elements of Dictyostelium discoideum amoeba have been visualized by light and electron microscopy in cells prepared with mixtures of glutaraldehyde and Triton-X-100. After negative staining, the peripheral regions of spreading amoebae show a complex meshwork of actin filaments, the majority of which were less than 0.25 microns in length. Multiple branch points, end to side abutments and cross-overs were characteristic features of the actin meshworks. Filopodia extending from the cell periphery consisted of bundles of actin filaments that penetrated into and merged with the actin meshworks in the spreading lamellae. Microtubules emanating from the nucleus associated body penetrated to differing extents into the actin meshworks, sometimes extending close to the cell periphery. Dictyostelium cytoskeletons prepared as described here should prove useful for further studies on the locomotory mechanism.