Lipid changes in the bones of the healing vitamin D-deficient phosphate-deficient rat.
Lipid changes in the bones of the healing vitamin D-deficient phosphate-deficient rat.
复制标题
维生素 D 缺乏、磷酸盐缺乏的愈合大鼠骨骼中的脂质变化。
DOI：
10.1016/8756-3282(85)90050-x
复制
发表时间：
1985
期刊：
影响因子：
4.1
通讯作者：
Boskey,AL
中科院分区：
文献类型：
--
作者：
Boskey,AL
维生素D缺乏、磷酸盐缺乏大鼠的骨骼具有与正常骨骼显著不同的脂质组成。具体来说，这些骨骼具有升高的胆固醇和降低的溶血磷脂和游离脂肪酸含量。用单剂量的维生素D和磷酸盐治疗这些动物在72小时内产生愈合，并导致生长板和松质骨脂质组成的改变的快速校正。影像学和组织学检查证实这些动物的佝偻病/骨软化状态愈合。组织形态学测量显示松质骨的相对类骨质体积迅速接近正常对照的7%值，从佝偻病动物的29%下降到12 h的16%和72 h的8.5%。在12 h时可检测到灰分重量、Ca：P比、微晶大小和完整性的显著变化，这些参数在72 h内接近正常动物中发现的值。钙-酸性磷脂-磷酸盐复合物，这是已知的，以促进羟基磷灰石的形成，在12小时在骨骺，松质骨和皮质骨的浓度达到峰值，迅速恢复到正常值后的时间。在未经处理的动物的复合酸性磷脂含量的非矿化骨骺是在正常的矿化骨骺，而复合物的含量减少在未经处理的动物的松质骨。
The bones of vitamin D-deficient, phosphate-deficient rats have a lipid composition that is significantly different from that of normal bones. Specifically, these bones have elevated cholesterol and reduced lysophosphatide and free fatty acid contents. Treatment of these animals with a single dose of vitamin D and phosphate produces healing within 72 h and causes rapid corrections of alterations in growth plate and cancellous bone lipid composition. Healing of the rachitic/osteomalacic state in these animals was demonstrated radiographically and histologically. Histomorphometric measurements showed that the relative osteoid volume of the cancellous bone rapidly approached the 7% value of normal controls, decreasing from 29% in the rachitic animals to 16% by 12 h and 8.5% by 72 h. Significant changes in ash weight, Ca:P ratio, and crystallite size and perfection were detectable at 12 h, with these parameters approaching values found in normal animals within 72 h. Calcium-acidic phospholipid-phosphate complexes, which are known to promote hydroxyapatite formation, peaked in concentration at 12 h in epiphysis, cancellous, and cortical bone, returning rapidly to normal values after that time. In untreated animals the complexed acidic phospholipid content of the nonmineralized epiphysis was comparable to that in normal mineralizing epiphysis, whereas the content of the complexes was reduced in the cancellous bones of the untreated animals.