Quartz crystal microbalance immunosensors for environmental monitoring
Quartz crystal microbalance immunosensors for environmental monitoring
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DOI：
10.1016/j.bios.2006.06.030
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发表时间：
2006-10-15
影响因子：
12.6
通讯作者：
Ishihara, Kazuhiko
中科院分区：
文献类型：
--
作者：
Kurosawa, Shigeru;Park, Jong-Won;Ishihara, Kazuhiko
本文讨论了石英晶体微天平（QCM）免疫传感器在环境监测中的应用。限制抗体分析问题的实际应用的因素也被提出。在QCM免疫传感器设备的几个候选者中，选择的QCM设备和振荡电路进行了彻底的测试和开发，以获得高度稳定和灵敏的频率信号。通过自组装单层膜技术和人工磷脂（2-甲基丙烯酰氧乙基磷酸胆碱（MPC））聚合物，设计并控制QCM免疫传感器的生物界面，将抗体固定在QCM表面，减少非特异性结合，抑制固定抗体的变性。加入MPC聚合物作为抗体稳定剂，以减少抗原溶液的非特异性结合，并稳定抗体固定QCM的免疫活性。此外，它提供了使用QCM免疫传感器检测和定量环境样品的例子。用该免疫传感器对粉煤灰提取样品中二恶英的分析结果表明，与GC/MS方法具有良好的相关性。竞争性免疫测定和信号增强步骤的整合方案用于使用QCM免疫传感器以极低的检测限检测低分子分析物。此外，当使用抗双酚A抗体缀合的MPC聚合物纳米颗粒时，通过信号增强步骤，其检测限从0.1扩展到0.01 ng/ml。QCM免疫传感器方法已证明其作为环境监测的替代筛选方法的有效性，因为这些结果与通过环境监测方法（如ELISA和GC/MS）获得的结果进行了比较。
This paper presents discussion of quartz crystal microbalance (QCM) immunosensors for environmental monitoring. Factors limiting the practical application of antibodies to analytical problems are also presented. Among several candidates for the QCM immunosensor device, selected QCM devices and oscillating circuits were tested thoroughly and developed to obtain highly stable and sensitive frequency signals. The biointerface of QCM immunosensor was designed and controlled to immobilize antibody on the QCM surface, to reduce non-specific binding and to suppress denaturation of immobilizing antibody by self-assembled monolayer technique and artificial phospholipid (2-methacryloyloxyethyl phosphorylcholine (MPC)) polymer. MPC polymer as a antibody-stabilizing reagent was added to reduce non-specific binding of the antigen solution and stabilize the immunologic activity of the antibody-immobilized QCM. In addition, it provides examples for detection and quantitation of environmental samples using QCM immunosensors. The analytical results for fly ash extracted samples of dioxins using the QCM immunosensor indicated a good relationship with GC/MS methods. The integrating protocols of the competitive immunoassay and signal-enhancing step are for detecting low molecular analytes with extremely low detection limits using an QCM immunosensor. Furthermore, its detect limitation was extended from 0.1 to 0.01 ng/ml by the signal-enhancing step when the anti-bisphenol-A antibody conjugated MPC polymeric nanoparticles was used. The QCM immunosensor method has demonstrated its effectiveness as an alternative screening method for environmental monitoring because these results were compared with results obtained through environmental monitoring methods such as ELISA and GC/MS. (c) 2006 Elsevier B.V. All rights reserved.