A new class of genetic element, staphylococcus cassette chromosome mec, encodes methicillin resistance in Staphylococcus aureus
A new class of genetic element, staphylococcus cassette chromosome mec, encodes methicillin resistance in Staphylococcus aureus
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DOI：
10.1128/aac.44.6.1549-1555.2000
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发表时间：
2000-06-01
影响因子：
4.9
通讯作者：
Hiramatsu, K
中科院分区：
文献类型：
--
作者：
Katayama, Y;Ito, T;Hiramatsu, K
我们先前已经证明，金黄色葡萄球菌菌株N315的甲氧西林耐药基因mecA位于插入染色体中的大（52-kb）DNA盒（称为葡萄球菌盒染色体mec [SCCmec]）内。通过整个DNA的序列测定，我们鉴定了两个新的基因（指定的盒式染色体重组酶基因[ccrA和ccrB]），其编码与转化酶/解离酶家族的重组酶具有部分同源性的多肽。发现开放阅读框催化从耐甲氧西林的S.金黄色葡萄球菌染色体和SCCmec的位点特异性以及方向特异性整合到S.当作为重组多拷贝质粒导入细胞时，金黄色葡萄球菌染色体的DNA片段被重组。我们建议，SCCmec驱动的一组新的重组酶代表了一个新的家庭的葡萄球菌基因组元件。
We have previously shown that the methicillin-resistance gene mecA of Staphylococcus aureus strain N315 is localized within a large (52-kb) DNA cassette (designated the staphylococcal cassette chromosome mec [SCCmec]) inserted in the chromosome. By sequence determination of the entire DNA, we identified two novel genes (designated cassette chromosome recombinase genes [ccrA and ccrB]) encoding polypeptides having a partial homology to recombinases of the invertase/resolvase family. The open reading frames were found to catalyze precise excision of the SCCmec from the methicillin-resistant S. aureus chromosome and site-specific as well as orientation-specific integration of the SCCmec into the S. aureus chromosome when introduced into the cells as a recombinant multicopy plasmid. We propose that SCCmec driven by a novel set of recombinases represents a new family of staphylococcal genomic elements.