Evidence for short-time divergence and long-time conservation of tissue-specific expression after gene duplication
Evidence for short-time divergence and long-time conservation of tissue-specific expression after gene duplication
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DOI：
10.1093/bib/bbr022
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发表时间：
2011-09-01
影响因子：
9.5
通讯作者：
Gabaldon, Toni
中科院分区：
文献类型：
--
作者：
Huerta-Cepas, Jaime;Dopazo, Joaquin;Gabaldon, Toni
基因复制是基因组获得新功能的主要机制之一。已经提出基因重复的保留可以与组织表达分歧的过程相关联。这些模型预测，收购不同的表达模式，应收购后不久的重复，并预计在组织表达的较大分歧旁系同源物，相比，直系同源物的相似年龄。许多研究表明，基因重复往往具有不同的表达模式，基因家族的扩展与高水平的组织特异性有关。然而，这些过程发生的时间框架很少被详细研究，特别是在脊椎动物中，并且大多数分析不包括直系同源物的直接比较，作为在没有重复的情况下预期组织特异性水平的基线。为了评估重复对表达分歧的具体贡献，我们在此结合联合收割机系统发育分析和来自人和小鼠的表达数据。特别是，我们研究了人类-小鼠旁系同源物之间的空间表达差异，特别是在哺乳动物辐射后复制，并将其与同一物种中的直系同源物进行比较。我们的研究结果表明，基因重复导致组织特异性水平的增加，这往往会发生后迅速重复事件。
Gene duplication is one of the main mechanisms by which genomes can acquire novel functions. It has been proposed that the retention of gene duplicates can be associated to processes of tissue expression divergence. These models predict that acquisition of divergent expression patterns should be acquired shortly after the duplication, and that larger divergence in tissue expression would be expected for paralogs, as compared to orthologs of a similar age. Many studies have shown that gene duplicates tend to have divergent expression patterns and that gene family expansions are associated with high levels of tissue specificity. However, the timeframe in which these processes occur have rarely been investigated in detail, particularly in vertebrates, and most analyses do not include direct comparisons of orthologs as a baseline for the expected levels of tissue specificity in absence of duplications. To assess the specific contribution of duplications to expression divergence, we combine here phylogenetic analyses and expression data from human and mouse. In particular, we study differences in spatial expression among human-mouse paralogs, specifically duplicated after the radiation of mammals, and compare them to pairs of orthologs in the same species. Our results show that gene duplication leads to increased levels of tissue specificity and that this tends to occur promptly after the duplication event.