Reconstruction of the basement membrane in a cultured submandibular gland
Reconstruction of the basement membrane in a cultured submandibular gland
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培养下颌下腺基底膜的重建
DOI：
--
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
S. Yamashina
中科院分区：
文献类型：
--
作者：
Y. Kadoya;S. Yamashina
总结在大鼠下颌下原基的第16天，层粘连蛋白（LM）和IV型胶原（Col-IV）被发现在所有情况下，共定位不仅在基底膜，而且在粗面内质网的上皮细胞，表明基底膜的成分的合成大大增强在这个特定的阶段，广泛的分支形成。使用16天胚胎的下颌下腺，模型系统被开发用于确定基底膜的结构组织。用胶原酶和分散酶消化预先存在的基底膜，使其完全消失。随后逐步重建一个真实的基底膜证实了电镜和免疫组化LM和ColIV。在模型系统中，这种恢复在培养4 h时开始，到8 h时形成完成。在恢复过程中，厚束的肌动蛋白丝出现在基底细胞质过渡。电子显微镜分析表明，两个前体结构，聚集的模糊纤维（1型细胞外基质（ECM））和10 nm厚的股桩（2型ECM），和一个真实的基底膜结构出现在膜重建的过程中。LM和Col-IV在这三种结构中总是位于一起。这些观察清楚地表明，前体，含有LM，Col-IV和最可能的硫酸乙酰肝素蛋白聚糖，似乎形成后立即分泌到细胞外空间，并组装成基底膜的刚性结构在8小时内。电镜和免疫组化结果显示，肾发育过程中基底膜的重建过程与肾小球基底膜的重建过程基本一致。因此，三阶段组装可能是基底膜形成的共同过程。
SummaryIn a rat submandibular rudiment on day 16, both laminin (LM) and type IV collagen (Col-IV) were found in all cases to colocalize not only in the basement membrane, but also in the rough endoplasmic reticulum of the epithelial cells, indicating that the synthesis of the components of basement membrane is greatly enhanced at this particular stage of extensive branch formation. Using the submandibular gland from a 16-day embryo, the model system was developed to determine the structural organization of the basement membrane. The pre-existing basement membrane was digested with collagenase and dispase, causing its complete disappearance. The subsequent gradual reconstruction of an authentic basement membrane was confirmed by electron microscopy and immunohistochemistry of LM and ColIV. In the model system, this recovery started at 4 h of culture, and formation was complete by 8 h. During the recovery, thick bundles of actin filaments appeared transitionally in the basal cytoplasm. Electron microscopic analysis indicated two precursor structures, aggregated fuzzy fibers (type 1 extracellular matrix (ECM)) and 10-nm-thick strand piles (type 2 ECM), and an authentic basement membrane structure appeared during the course of membrane reconstruction. LM and Col-IV were always located together in these three structures. These observations clearly indicate that the precursors, containing LM, Col-IV and most likely heparan sulfate proteoglycan, appeared to form immediately following their secretion into the extracellular space, and assembled into the rigid structure of basement membrane within 8 h. The ultrastructural and immunohistochemical process of basement membrane reconstruction appeared to coincide closely with that of the glomerular basement membrane in developing kidney. The three-stage assembly may therefore be a common process of basement membrane formation.
DOI：
10.1002/aja.1001810403
发表时间：
1988
期刊：
The American journal of anatomy
影响因子：
--
作者：
Inoue,S;Leblond,CP
通讯作者：
Leblond,CP
对各种基底膜中的层粘连蛋白、IV 型胶原蛋白和硫酸乙酰肝素蛋白多糖进行免疫金定量。
DOI：
10.1177/36.3.2963856
发表时间：
1988
期刊：
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
影响因子：
--
作者：
Grant,DS;Leblond,CP
通讯作者：
Leblond,CP