A comparison between two brine shrimp assays to detect in vitro cytotoxicity in marine natural products.
A comparison between two brine shrimp assays to detect in vitro cytotoxicity in marine natural products.
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DOI：
10.1186/1472-6750-2-17
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发表时间：
2002-09-23
影响因子：
3.5
通讯作者：
García-Grávalos MD
中科院分区：
文献类型：
--
作者：
Carballo JL;Hernández-Inda ZL;Pérez P;García-Grávalos MD
卤水对虾的致死率测定被认为是初步毒性评估的有用工具。它也被建议用于筛选植物提取物中的药理活性。然而，我们认为，在将卤虾方法用作测试天然海洋产品药理活性的一般生物测定之前，有必要对这些方法的适用性进行评估。对14种海洋无脊椎动物和6种大型藻类的异丙醇(2-PROH)提取物进行了对虾致死性测定(致死性测定)和另一种基于孵化抑制作用的测定(孵化率测定)。此外，还测定了提取物对肺癌A-549和结肠癌HT-29两种细胞株的细胞毒性，以评估虾类检测细胞毒活性的敏感性。两种海绵(Hyatella sp.，Dyside a sp.)，两种柳珊瑚(Pacifigorgia adamsii，Muricea sp.)，一种被囊动物(PolyClinum Laxum)，以及三种棘皮动物(Holothuria impatiens，Pseudoconus calfornica和Pharia parmidata)表现出很强的细胞抑制和细胞毒作用。孵化率测定表明，对两种人体细胞株(Hyatella sp.、Dyside a sp.、Pacifigorgia adamsii和Muricea sp.)有较强的活性，致死率测定也表明其中4种(Pacifigorgia adamsii、Muricea sp.、PolyClinum laxum和Pharia Pramidata)具有较高的致死率。每种生物检测方法都检测到了50%被认为对两个被测试的人类细胞株具有活性的物种的活性。然而，同时使用两种生物检测方法将这一比例提高到75%。我们的结果似乎与之前在植物提取物中建立的细胞毒性和卤虾致死率之间的相关性是一致的。我们建议同时使用这两种生物检测方法来测试天然海洋产品的药理活性。
The brine shrimp lethality assay is considered a useful tool for preliminary assessment of toxicity. It has also been suggested for screening pharmacological activities in plant extracts. However, we think that it is necessary to evaluate the suitability of the brine shrimp methods before they are used as a general bio-assay to test natural marine products for pharmacological activity. The bioactivity of the isopropanolic (2-PrOH) extracts of 14 species of marine invertebrates and 6 species of macroalgae was evaluated with the shrimp lethality assay (lethality assay), as well as with another assay based on the inhibition of hatching of the cyst (hatchability assay). The extracts were also assayed for cytotoxicity against two human cell lines, lung carcinoma A-549 and colon carcinoma HT-29, in order to assess the sensitivity of the shrimp assays to detect cytotoxic activity. Two sponges (Hyatella sp, Dysidea sp.), two gorgonians (Pacifigorgia adamsii, Muricea sp.), one tunicate (Polyclinum laxum), and three echinoderms (Holothuria impatiens, Pseudoconus californica and Pharia pyramidata) showed a strong cytostatic (growth inhibition) and cytotoxic effect. The hatchability assay showed a strong activity in 4 of the species active against the two human cell lines tested (Hyatella sp, Dysidea sp., Pacifigorgia adamsii and Muricea sp.), and the lethality assay also showed a high lethality in 4 of them (Pacifigorgia adamsii, Muricea sp., Polyclinum laxum, and Pharia pyramidata). Each bioassay detected activity in 50% of the species that were considered active against the two human cell lines tested. However, the simultaneous use of both bioassays increased the percentage to 75%. Our results seem consistent with the correlation previously established between cytotoxicity and brine shrimp lethality in plant extracts. We suggest using both bioassays simultaneously to test natural marine products for pharmacological activity.