IDENTIFICATION OF COPROGEN-B AND ITS BREAKDOWN PRODUCTS FROM HISTOPLASMA-CAPSULATUM
IDENTIFICATION OF COPROGEN-B AND ITS BREAKDOWN PRODUCTS FROM HISTOPLASMA-CAPSULATUM
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DOI：
10.1128/iai.35.3.990-996.1982
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发表时间：
1982-01-01
影响因子：
3.1
通讯作者：
BURT, WR
中科院分区：
文献类型：
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作者：
BURT, WR
添加到化学成分确定的液体介质中的 Fe 可抑制 37°C 下异羟肟酸的产生。 C 为 H. capsulatum。通过提取和通过纤维素磷酸盐（0.35%甲酸）的阳离子交换色谱法分离存在于荚膜梭菌培养后的低铁液体中的四种异羟肟酸，HA-I、HA-II、HA-III和HA-IV。制备的异羟肟酸铁的可见光谱表明HA-II和HA-III为单异羟肟酸盐； HA-I和HA-IV分别被鉴定为二异羟肟酸酯和三异羟肟酸酯。 HA-I（分离出的主要异羟肟酸）的还原水解产生鸟氨酸。 HA-IV 在水或 0.1 N NaOH 中水解导致形成 HA-I（二异羟肟酸）和 HA-II（单异羟肟酸）。根据纸电泳测定的 pH 5.2 和 2 时的电荷、TLC 上的 Rf 值、红外光谱以及对茚三酮的反应性，分离出的 3 种异羟肟酸被鉴定为去铁原 B (HA-IV) 及其分解产物二聚酸 (HA-I) 和反式镰刀菌宁 (HA-II)。 HA-I和HA-IV对荚膜梭菌的酵母和菌丝体形式的生长均表现出生长因子活性。
Fe added to a chemically defined liquid medium suppressed hydroxamic acid production at 37.degree. C by H. capsulatum. Four hydroxamic acids, HA-I, HA-II, HA-III and HA-IV, present in the low-Fe fluid after the culture of H. capsulatum were isolated by extraction and cation-exchange chromatography through cellulose phosphate (0.35% formic acid). Visible spectra of prepared ferrihydroxamates indicated that HA-II and HA-III were monohydroxamates; HA-I and HA-IV were identified as di- and trihydroxamates, respectively. Reductive hydrolysis of HA-I (the major hydroxamic acid isolated) yielded ornithine. Hydrolysis of HA-IV in water or in 0.1 N NaOH resulted in the formation of HA-I (dihydroxamic acid) and HA-II (monohydroxamic acid). Based on their charge at pH 5.2 and 2 determined by paper electrophoresis, Rf values on TLC, IR spectra and reactivity to ninhydrin, 3 of the isolated hydroxamic acids were identified as deferricoprogen B (HA-IV) and its breakdown products, dimerumic acid (HA-I) and trans fusarinine (HA-II). HA-I and HA-IV exhibited growth factor activity for both yeast and mycelial forms of growth of H. capsulatum.