LncRNA IMFNCR Promotes Intramuscular Adipocyte Differentiation by Sponging miR-128-3p and miR-27b-3p
LncRNA IMFNCR Promotes Intramuscular Adipocyte Differentiation by Sponging miR-128-3p and miR-27b-3p
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LncRNA IMFNCR 通过海绵 miR-128-3p 和 miR-27b-3p 促进肌内脂肪细胞分化
DOI：
10.3389/fgene.2019.00042
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发表时间：
2019-02-11
影响因子：
3.7
通讯作者：
Sun, Gui-rong
中科院分区：
文献类型：
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作者：
Zhang, Meng;Li, Fang;Sun, Gui-rong
家禽肉质受多种因素影响，其中肌内脂肪（IMF）占主导地位。 IMF含量影响鸡肉的嫩度、多汁性和风味。越来越多的研究关注 lncRNA 在脂肪细胞分化中的功能。然而，人们对与肌内脂肪细胞分化相关的lncRNA知之甚少。在本研究中，我们关注肌内脂肪分化过程中上调的lncRNA，我们将其命名为肌内脂肪相关长非编码RNA（IMFNCR）。 IMFNCR 促进肌内脂肪细胞分化。深入分析表明，IMFNCR 作为 miR-128-3p 和 miR-27b-3p 的分子海绵，而 PPARG 是鸡中 miR-128-3p 和 miR-27b-3p 的直接靶标。高脂肪、高蛋白日粮抑制鸡体内IMFNCR水平。此外，IMFNCR 水平与鸡胸肌组织中 PPARG mRNA 水平呈正相关，这是 ceRNA 功能的重要推论。总之，我们的研究表明 IMFNCR 作为 ceRNA 隔离 miR-128-3p 和 miR-27b-3p，导致 PPARG 表达升高，从而促进肌内脂肪细胞分化。总而言之，我们的研究结果可能有助于更深入地了解鸡肉 IMF 沉积和改善禽肉质量。
Poultry meat quality is affected by many factors, among which intramuscular fat (IMF) is predominant. IMF content affects the tenderness, juiciness, and flavor of chicken. An increasing number of studies are focusing on the functions of lncRNAs in adipocyte differentiation. However, little is known about lncRNAs associated with intramuscular adipocyte differentiation. In the present study, we focused on an up-regulated lncRNA during intramuscular adipogenetic differentiation, which we named intramuscular fat-associated long non-coding RNA (IMFNCR). IMFNCR promotes intramuscular adipocyte differentiation. In-depth analyses showed that IMFNCR acts as a molecular sponge for miR-128-3p and miR-27b-3p and that PPARG is a direct target of miR-128-3p and miR-27b-3p in chicken. High-fat and high-protein diet inhibited chicken IMFNCR level in vivo. Moreover, IMFNCR level was positively correlated with PPARG mRNA level in chicken breast muscle tissues, a vital corollary to ceRNA function. Altogether, our research showed that IMFNCR acts as a ceRNA to sequester miR-128-3p and miR-27b-3p, leading to heightened PPARG expression, and thus promotes intramuscular adipocyte differentiation. Taken together, our findings may contribute to a more thorough understanding of chicken IMF deposition and the improvement of poultry meat quality.