Solid structure and biodegradation of the compositionally fractionated poly(3-hydroxybutyric acid-co-3-hydroxypropionic acid)s
Solid structure and biodegradation of the compositionally fractionated poly(3-hydroxybutyric acid-co-3-hydroxypropionic acid)s
复制标题
成分分级聚(3-羟基丁酸-co-3-羟基丙酸)的固体结构和生物降解
DOI：
10.1016/s0032-3861(99)00044-0
复制
发表时间：
1999
期刊：
影响因子：
4.6
通讯作者：
Y. Inoue
中科院分区：
文献类型：
--
作者：
Amin Cao;Y. Arai;N. Yoshie;K. Kasuya;Y. Doi;Y. Inoue
研究了组成分级的细菌聚（3-羟基丁酸-co-3-羟基丙酸）[P（3 HB-co-3 HP）]的固体结构和生物降解行为。利用从粪产碱杆菌（Alcaligenes faecalis）T1菌株中分离纯化的胞外聚3-羟基丁酸[P（3 HB）]解聚酶，研究了P（3 HB）、聚3-羟基丙酸[P（3 HP）]以及分离后的P（3 HB-co-3 HP）s的降解行为。结果表明，P（3 HB）和P（3 HP）的酶降解特性具有明显的解聚酶浓度依赖性。对于分级共聚酯，结果表明，具有较高的3 HB含量的P（3 HB-co-3 HP）s，形成P（3 HB）型微晶，表现出比具有较高的3 HP含量的共聚酯，形成P（3 HP）型微晶的高得多的酶降解速率，并且无定形淀粉几乎不能被降解。酶解产物的1HNMR分析表明，P（3 HB）首先降解为二聚体，然后降解为单体。与此相反，低聚物的存在下，高于二聚体的P（3 HP）的降解产物中，和3 HP含量依赖性的降解产物的分馏P（3 HB-co-3 HP）的确认。此外，还采用BOD法对河水中的环境可降解性进行了评价。结果证实了P（3 HB）和共聚酯的环境降解性，除了P（3 HP），表明3 HB共聚单体对共聚酯中的3 HP单元的生物降解的诱导作用。
The solid structure and biodegradation behavior of the compositionally fractionated bacterial poly(3-hydroxybutyric acid-co-3-hydroxypropionic acid)s [P(3HB-co-3HP)] were examined. Utilizing the extracellular poly(3-hydroxybutyric acid) [P(3HB)]depolymerase purified from the bacterium strain Alcaligenes faecalis T1, enzymatic degradation behavior was investigated for P(3HB), poly(3-hydroxypropionic acid) [P(3HP)]and the fractionated P(3HB-co-3HP)s. It was revealed that P(3HB) and P(3HP) showed the distinguishable depolymerase concentration dependence of enzymatic degradation feature. For the fractionated copolyesters, the results indicated that P(3HB-co-3HP)s with higher 3HB contents which form P(3HB)-type of crystallites exhibited much higher enzymatic degradation rates than those of copolyesters with higher 3HP contents which form P(3HP)-type of crystallites, and that the amorphous copolyester could hardly be degraded. The1H NMR analyses of the enzymatic degradation products revealed that P(3HB) was first degraded into dimers and then into monomers. In contrast, the presence of oligomers higher than dimers was suggested in the degradation products of P(3HP), and the 3HP content dependence of degradation products was confirmed for the fractionated P(3HB-co-3HP)s. In addition, environmental degradabilities were assessed by BOD method in the river water. The results confirmed the environmental degradabilities of P(3HB) and copolyesters except for P(3HP), indicating the inducing effect of 3HB comonomers on the biodegradation of 3HP units in copolyesters.