The relationship between anion exchange and net anion flow across the human red blood cell membrane.
The relationship between anion exchange and net anion flow across the human red blood cell membrane.
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DOI：
10.1085/jgp.69.3.363
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发表时间：
1977-03
期刊：
影响因子：
--
通讯作者：
Rothstein A
中科院分区：
文献类型：
--
作者：
Knauf PA;Fuhrmann GF;Rothstein S;Rothstein A
人红细胞的传导性（净）阴离子渗透性由高缬氨霉素浓度下的KCl或K2 SO 4净流出到低K+介质中确定，在该条件下，盐流出主要受净阴离子渗透性限制。二磺酸芪，抑制剂的阴离子交换，也抑制KCl或K2 SO 4在这些条件下流出，但在较低的缬氨霉素浓度，其中K+渗透性是主要的限制因素是有效性较低。不同浓度的4，4 '-二异硫氰基二苯乙烯-2，2'-二磺酸盐（DIDS）对净硫酸盐通量和交换硫酸盐通量的抑制作用相似，两者几乎完全对DIDS敏感。在Cl-的情况下，一个高度的相关性也被发现之间的抑制净和交换通量，但在这种情况下，约35%的净通量是不敏感的DIDS。净传递过程和交换传递过程在阴离子选择性上有显著的不同。净氯离子渗透率仅为净硫酸盐渗透率的四倍，而氯离子交换比硫酸盐交换快10，000倍以上。净OH-渗透性，由类似的方法确定，是超过四个数量级大于Cl-，但也是敏感的DIDS。这些数据和其他人进行了讨论的可能性，一个共同的元素可能涉及净和交换阴离子运输。
The conductive (net) anion permeability of human red blood cells was determined from net KCl or K2SO4 effluxes into low K+ media at high valinomycin concentrations, conditions under which the salt efflux is limited primarily by the net anion permeability. Disulfonic stilbenes, inhibitors of anion exchange, also inhibited KCl or K2SO4 efflux under these conditions, but were less effective at lower valinomycin concentrations where K+ permeability is the primary limiting factor. Various concentrations of 4,4'-diisothiocyanostilbene-2,2'-disulfonate (DIDS) had similar inhibitory effects on net and exchange sulfate fluxes, both of which were almost completely DIDS sensitive. In the case of Cl-, a high correlation was also found between inhibition of net and exchange fluxes, but in this case about 35% of the net flux was insensitive to DIDS. The net and exchange transport processes differed strikingly in their anion selectivity. Net chloride permeability was only four times as high as net sulfate permeability, whereas chloride exchange is over 10,000 times faster than sulfate exchange. Net OH- permeability, determined by an analogous method, was over four orders of magnitude larger than that of Cl-, but was also sensitive to DIDS. These data and others are discussed in terms of the possibility that a common element may be involved in both net and exchange anion transport.