AN ISOFORM OF PROTEIN DISULFIDE-ISOMERASE IS EXPRESSED IN THE DEVELOPING ACROSOME OF SPERMATIDS DURING RAT SPERMIOGENESIS AND IS TRANSPORTED INTO THE NUCLEUS OF MATURE SPERMATIDS AND EPIDIDYMAL SPERMATOZOA
AN ISOFORM OF PROTEIN DISULFIDE-ISOMERASE IS EXPRESSED IN THE DEVELOPING ACROSOME OF SPERMATIDS DURING RAT SPERMIOGENESIS AND IS TRANSPORTED INTO THE NUCLEUS OF MATURE SPERMATIDS AND EPIDIDYMAL SPERMATOZOA
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DOI：
10.1007/bf00267822
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发表时间：
1993-12-01
期刊：
影响因子：
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通讯作者：
MIURA, AB
中科院分区：
文献类型：
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作者：
OHTANI, H;WAKUI, H;MIURA, AB
我们从大鼠肝脏中纯化了一种蛋白质二硫键异构酶（EC 5.3.4.1），并在家兔体内制备了抗该蛋白质的特异性抗体。免疫组织化学研究，使用这种抗体对大鼠睾丸切片，在光镜和电镜水平，显示了特定的定位蛋白质二硫键异构酶的亚型在精子细胞的顶体发育。该蛋白在高尔基体晚期从高尔基体转移到顶体囊泡中，并在帽状期、顶体期和成熟期保留在精子细胞的顶体中。成熟期精子细胞除顶体外，还出现在细胞核中，附睾精子细胞的细胞核中也有表达。通过免疫印迹分析，发现睾丸中蛋白质二硫键异构酶的几乎所有亚型都可被等渗缓冲液提取。相反，附睾中的蛋白质完全溶解需要去污剂提取。这些结果表明，蛋白二硫键异构酶亚型是一种新的顶体内可溶性蛋白，该蛋白开始进入睾丸成熟精子细胞的细胞核，并与附睾精子的核成分紧密结合。
We have purified an isoform of protein disulfide isomerase (EC 5.3.4.1) from rat liver, and raised a specific antibody against the purified protein in rabbit. Immunohistochemical studies using this antibody on rat testis sections, at both light and electron microscopic levels, showed a specific localization of the isoform of protein disulfide isomerase in the developing acrosome of the spermatids. The protein was transferred to the acrosomic vesicle from the Golgi apparatus at late Golgi phase, and remained present in the acrosome of spermatids during cap phase, acrosome phase, and maturation phase. In addition to the acrosome, the protein appeared in the nucleus of spermatids during maturation phase, and was localized in the nucleus of epididymal spermatozoa. By immunoblot analysis, almost all of the isoform of protein disulfide isomerase in the testis was found to be extractable by an isotonic buffer. On the contrary, detergent extraction was required for complete solubilization of the protein in the epididymis. These results suggest that the isoform of protein disulfide isomerase is a new intra-acrosomal soluble protein, and that the protein begins to enter the nucleus of mature spermatids in the testis and tightly binds to the nuclear components in epididymal spermatozoa.