Fibronectin on circulating extracellular vesicles as a liquid biopsy to detect breast cancer.
Fibronectin on circulating extracellular vesicles as a liquid biopsy to detect breast cancer.
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DOI：
10.18632/oncotarget.9561
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发表时间：
2016-06-28
期刊：
影响因子：
--
通讯作者：
Baek MC
中科院分区：
文献类型：
--
作者：
Moon PG;Lee JE;Cho YE;Lee SJ;Chae YS;Jung JH;Kim IS;Park HY;Baek MC
从癌细胞分泌的细胞外囊泡（EV）具有产生癌症生物标志物特征的潜力。由于纤连蛋白（FN）存在于人乳腺癌细胞系分泌的EV表面，因此被选为候选生物标志物。随后的研究使用两种类型的酶联免疫吸附测定（ELISA）来确定来自无病个体（n=70）、BC患者（n=240）、手术切除后的BC患者（n=40）、良性乳腺肿瘤患者（n=55）和非癌性疾病患者的血浆样品中这些蛋白质的存在（甲状腺炎、胃炎、B型肝炎和类风湿性关节炎; n=80）。FN水平显著升高（p<. 0001），并在肿瘤切除后恢复正常。细胞外囊泡中FN检测的诊断准确性（ELISA方法1）（曲线下面积0.81; 95%CI 0.76 ~ 0.86;敏感性65.1%，特异性83.2%）也优于血浆FN检测（ELISA方法2）（曲线下面积，0.77; 95%CI，0.72至0.83;灵敏度为69.2%，特异性为73.3%）。血浆FN的诊断准确性在早期BC和所有BC患者中以及在两组中相似。这种检测循环EV上FN的液体活检可能是检测早期乳腺癌的有希望的方法。
Extracellular vesicles (EVs) secreted from cancer cells have potential for generating cancer biomarker signatures. Fibronectin (FN) was selected as a biomarker candidate, due to the presence in surface on EVs secreted from human breast cancer cell lines. A subsequent study used two types of enzyme-linked immunosorbent assays (ELISA) to determine the presence of these proteins in plasma samples from disease-free individuals (n=70), patients with BC (n=240), BC patients after surgical resection (n=40), patients with benign breast tumor (n=55), and patients with non-cancerous diseases (thyroiditis, gastritis, hepatitis B, and rheumatoid arthritis; n=80). FN levels were significantly elevated (p<. 0001) at all stages of BC, and returned to normal after tumor removal. The diagnostic accuracy for FN detection in extracellular vesicles (ELISA method 1) (area under the curve, 0.81; 95% CI, 0.76 to 0.86; sensitivity of 65.1% and specificity of 83.2%) were also better than those for FN detection in the plasma (ELISA method 2) (area under the curve, 0.77; 95% CI, 0.72 to 0.83; sensitivity of 69.2% and specificity of 73.3%) in BC. The diagnostic accuracy of plasma FN was similar in both the early-stage BC and all BC patients, as well as in the two sets. This liquid biopsy to detect FN on circulating EVs could be a promising method to detect early breast cancer.
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