Region-specific neutralization of Indian cobra (Naja naja) venom by polyclonal antibody raised against the eastern regional venom:: A comparative study of the venoms from three different geographical distributions
Region-specific neutralization of Indian cobra (Naja naja) venom by polyclonal antibody raised against the eastern regional venom:: A comparative study of the venoms from three different geographical distributions
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DOI：
10.1016/j.intimp.2006.08.014
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发表时间：
2007-01-01
影响因子：
5.6
通讯作者：
Kemparaju, K.
中科院分区：
文献类型：
--
作者：
Shashidharamurthy, R.;Kemparaju, K.
来自不同地理位置的印度眼镜蛇（Naja Naja）毒液的组成，生化和药理学特性各不相同。在这项研究中比较了来自东部，西部和南部的毒液样品。发现来自东部地区的毒液是三种区域毒液中最致命的。发现针对东毒液制备的单价抗蛇毒（NNEV-IGG）与其他两个区域毒液进行了反应。 nnev-igg的Ag：1:25的Ab比完全中和东毒液的致死性。在此比例下，它没有中和其他两个毒液，但是实验小鼠的生存时间显着延长。市售的多价抗蛇毒抗性中和西部毒液的致死性在1:60的Ag：Ab比率增加，并增加了注入了东部和南部毒液缘的实验小鼠的生存时间。此外，NNEV-IGG中和所有三种毒液样品的测试药理和酶促活性依赖性剂量，中和效力随测试毒液的地理起源而变化。因此，本研究证明了来自不同地理区域的毒液的免疫学特性的多样性，并强调了发展区域特异性抗蛇毒对治疗目的的重要性。 （c）2006年由Elsevier B.V.出版
Indian cobra (Naja naja) venoms from different geographical locations vary in their composition, biochemical, and pharmacological properties. Venom samples from eastern, western and southern India are compared in this study. The venom from eastern region was found to be the most lethal of the three regional venoms. Monovalent antivenom (NNEV-IgG) prepared against the eastern venom was found to cross-react with the other two regional venoms. NNEV-IgG at an Ag:Ab ratio of 1:25 completely neutralized the lethality of eastern venom. At this ratio, it did not neutralize the other two venoms, but the survival time of experimental mice was extended significantly. Commercially available polyvalent antivenom neutralized the lethality of western venom at an Ag:Ab ratio of 1:60 and increased the survival time of experimental mice injected with eastern and southern venoms marginally. Further, NNEV-IgG neutralized the tested pharmacological and enzymatic activities of all the three venom samples dose dependently, with neutralization potency varying with the geographic origin of the tested venoms. Thus, the present study demonstrates the diversity in the immunological properties of venom from different geographical regions and underscores the importance of developing region-specific antivenoms for therapeutic purpose. (c) 2006 Published by Elsevier B.V.