Differences in Laryngeal Neurotrophic Factor Gene Expression after Recurrent Laryngeal Nerve and Vagus Nerve Injuries
Differences in Laryngeal Neurotrophic Factor Gene Expression after Recurrent Laryngeal Nerve and Vagus Nerve Injuries
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DOI：
10.1177/000348941312201009
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发表时间：
2013-10
期刊：
影响因子：
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通讯作者：
Stacey Halum;Khadijeh Bijangi‐Vishehsaraei;M. Saadatzadeh;Bryan R. McRae
中科院分区：
文献类型：
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作者：
Stacey Halum;Khadijeh Bijangi‐Vishehsaraei;M. Saadatzadeh;Bryan R. McRae
目的喉返神经(RLN)和迷走神经(VN)损伤后均有不同程度的自发神经支配，但喉肌神经营养因子(NF)在RLN和VN损伤后的表达情况尚不清楚。本研究的目的是研究RLN或VN损伤后喉肌中5种典型神经营养因子基因表达的时间依赖性变化，以及这些变化与肌电检测的神经支配状态之间的对应关系。方法36只雄性大鼠接受左侧RLN切断(12只)、左侧VN切断(12只)或假手术(12只)。主要结果包括3天和1个月的肌电评估和逆转录-聚合酶链式反应定量喉肌核因子的表达。结果3d肌电图显示VN损伤组无电活动，假手术组活动正常，RLN损伤组新生单位募集减少。逆转录-聚合酶链式反应显示，不同类型的神经损伤(RLN或VN)和特定的喉肌(环鳗后肌或内收肌)，喉肌肉中的NF基因表达的变化不同。结论颅神经X伤后喉肌神经营养因子的表达变化与神经损伤程度和受累肌肉有关。
Objectives Recurrent laryngeal nerve (RLN) and vagus nerve (VN) injuries characteristically are followed by differing degrees of spontaneous reinnervation, yet laryngeal muscle neurotrophic factor (NF) expression profiles after RLN and VN injuries have not been well elucidated. This study's objective was to determine the relative changes in gene expression of 5 well-characterized NFs from laryngeal muscle after RLN or VN injuries in a time-dependent fashion, and demonstrate how these changes correspond with electromyography-assessed innervation status. Methods Thirty-six male rats underwent left RLN transection (12 rats), left VN transection (12 rats), or a sham procedure (12 rats). The primary outcomes included electromyographic assessment and laryngeal muscle NF expression quantification with reverse transcription polymerase chain reaction at 3 days and at 1 month. Results Electromyography at 3 days demonstrated electrical silence in the VN injury group, normal activity in the sham group, and nascent units with decreased recruitment in the RLN injury group. Reverse transcription polymerase chain reaction demonstrated that changes in NF gene expression from laryngeal muscles varied depending on the type of nerve injury (RLN or VN) and the specific laryngeal muscle (posterior cricoarytenoid or adductor) assessed. Conclusions Laryngeal muscle NF expression profiles after cranial nerve X injury depend both upon the level of nerve injury and upon the muscles involved.