Multicoloured fluorescent indicators for live-cell and in vivo imaging of inorganic mercury dynamics
Multicoloured fluorescent indicators for live-cell and in vivo imaging of inorganic mercury dynamics
复制标题
用于无机汞动力学活细胞和体内成像的彩色荧光指示剂
DOI：
10.1016/j.freeradbiomed.2018.04.562
复制
发表时间：
2018-06-01
影响因子：
7.4
通讯作者：
Yang，Yi
中科院分区：
文献类型：
--
作者：
Tao，Rongkun;Shi，Mei;Yang，Yi
工程荧光指示剂用于可视化汞离子（Hg 2+）是有力的工具，说明细胞内的分布和严重的毒性离子。然而，活细胞和体内Hg 2+的灵敏度和特异性检测是具有挑战性的。本文报道了通过将环状排列的荧光蛋白插入高度汞特异性的阻遏物中，开发了绿色或红色的汞离子荧光指示剂。这些传感器提供了一个快速，灵敏，特异，实时读出的Hg2+在溶液中，细菌，哺乳动物细胞的亚细胞器，和斑马鱼的动态，从而提供了一个有用的新方法，Hg2+检测和生物成像。结合过氧化氢传感器Hyper，我们发现汞的摄入会在单细胞水平上引发亚细胞氧化事件，并提供了汞和氧化损伤因果关系的视觉证据。这些传感器将描绘出汞对细胞功能的毒性。
Engineered fluorescent indicators for visualizing mercury ion (Hg2+) are powerful tools to illustrate the intracellular distribution and serious toxicity of the ion. However, the sensitive and specific detection of Hg2+in living cells and in vivo is challenging. This paper reported the development of fluorescent indicators for Hg2+in green or red color by inserting a circularly permuted fluorescent protein into a highly mercury-specific repressor. These sensors provided a rapid, sensitive, specific, and real-time read-out of Hg2+dynamics in solutions, bacteria, subcellular organelles of mammalian cells, and zebrafish, thereby providing a useful new method for Hg2+detection and bioimaging. In conjunction with the hydrogen peroxide sensor HyPer, we found mercury uptake would trigger subcellular oxidative events at the single-cell level, and provided visual evidence of the causality of mercury and oxidative damage. These sensors would paint the landscape of mercury toxicity to cell functions.