Molecular Weight Measurements of Low Molecular Weight Heparins by Gel Permeation Chromatography
Molecular Weight Measurements of Low Molecular Weight Heparins by Gel Permeation Chromatography
复制标题
通过凝胶渗透色谱法测量低分子量肝素的分子量
DOI：
--
复制
发表时间：
1997
影响因子：
6.7
通讯作者：
T. Barrowcliffe
中科院分区：
文献类型：
--
作者：
B. Mulloy;C. Gee;S. Wheeler;R. Wait;E. Gray;T. Barrowcliffe
采用高效凝胶渗透色谱法测定了低分子量肝素样品的分子量分布，并将肝素酶降解物质（90/686）作为校准剂，作为第一种国际标准制剂（IRP）低分子量肝素的分子量校准。描述了该校准剂作为广泛分子量标准的使用，并根据在一个实验室收集的数年数据提供了校准表。为了确定校准剂中被溶解的寡糖峰的聚合度分配，从第1 IRP分离的寡糖分子量通过快速原子轰击质谱（FAB MS）独立测定。对市售低分子量肝素的分子量分布进行了表征。从几个来源测量的肝素分子量标准的分子量参数提供了本研究和其他研究的分子量尺度之间的比较。
Summary The molecular weight profiles of low molecular weight heparin samples have been measured by high-performance gel permeation chromatography using as calibrant the heparinase-degraded material (90/686) now established as the 1st International Reference Preparation (IRP) Low Molecular Weight Heparin for Molecular Weight Calibration. Use of the calibrant as a broad molecular weight standard is described and a calibration table provided based on data collected over several years in one laboratory. In order to confirm the assignment of degree of polymerisation to resolved oligosaccharide peaks in the calibrant, molecular weights of oligosaccharides fractionated from the 1st IRP were independently determined by fast atom bombardment mass spectrometry (FAB MS). The molecular weight distributions of commercial low molecular weight heparins have been characterized. Measurements of molecular weight parameters of heparin molecular weight standards from several sources provide comparisons between the molecular weight scales of this and other studies.