Metabolite Profile of Cervicovaginal Fluids from Early Pregnancy Is Not Predictive of Spontaneous Preterm Birth.
Metabolite Profile of Cervicovaginal Fluids from Early Pregnancy Is Not Predictive of Spontaneous Preterm Birth.
复制标题
妊娠早期宫颈阴道液的代谢物谱并不能预测自发性早产。
DOI：
10.3390/ijms161126052
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发表时间：
2015-11-19
影响因子：
5.6
通讯作者：
Baker PN
中科院分区：
文献类型：
--
作者：
Thomas MM;Sulek K;McKenzie EJ;Jones B;Han TL;Villas-Boas SG;Kenny LC;McCowan LM;Baker PN
在我们的研究中，我们使用了基于质谱学的代谢组学方法来寻找可以作为自发早产(SPTB)早期指标的生物标志物。样本是从新西兰奥克兰的妊娠终点筛查(SCOPE)生物库中选择的嵌套式病例对照研究。宫颈阴道拭子是在20周时从最初被评估为sPTB低风险的妇女身上收集的。样品用气相色谱-质谱仪(GC-MS)分析。尽管生物量很低(16-23毫克)，但检测到112种化合物。统计学分析显示与sPTB无显著相关性。对妊娠早期报告的感染和血浆炎症标志物的比较显示，两个炎症标志物与报告的感染相关，但与代谢物图谱中的任何化合物都没有相关性。我们推测，宫颈阴道液代谢组中缺乏sPTB的生物标志物仅仅是因为它在怀孕早期缺乏这样的标志物。我们建议研究替代生物体液作为肺结核的标志物。我们的结果导致我们呼吁对先前发表的与宫颈阴道液中sPTB生物标记物相关的代谢组学数据进行更严格的审查，因为使用小、高风险或晚期妊娠队列可能识别与正常人群风险预测无关的代谢物生物标记物。
In our study, we used a mass spectrometry-based metabolomic approach to search for biomarkers that may act as early indicators of spontaneous preterm birth (sPTB). Samples were selected as a nested case-control study from the Screening for Pregnancy Endpoints (SCOPE) biobank in Auckland, New Zealand. Cervicovaginal swabs were collected at 20 weeks from women who were originally assessed as being at low risk of sPTB. Samples were analysed using gas chromatography-mass spectrometry (GC-MS). Despite the low amount of biomass (16–23 mg), 112 compounds were detected. Statistical analysis showed no significant correlations with sPTB. Comparison of reported infection and plasma inflammatory markers from early pregnancy showed two inflammatory markers were correlated with reported infection, but no correlation with any compounds in the metabolite profile was observed. We hypothesise that the lack of biomarkers of sPTB in the cervicovaginal fluid metabolome is simply because it lacks such markers in early pregnancy. We propose alternative biofluids be investigated for markers of sPTB. Our results lead us to call for greater scrutiny of previously published metabolomic data relating to biomarkers of sPTB in cervicovaginal fluids, as the use of small, high risk, or late pregnancy cohorts may identify metabolite biomarkers that are irrelevant for predicting risk in normal populations.