Evaluation of the indirect fluorescent antibody test for diagnosis of Babesia gibsoni infections in dogs.
Evaluation of the indirect fluorescent antibody test for diagnosis of Babesia gibsoni infections in dogs.
复制标题
间接荧光抗体试验诊断犬吉布氏巴贝虫感染的评价。
DOI：
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发表时间：
1993
影响因子：
1
通讯作者：
Patricia A. Conrad
中科院分区：
文献类型：
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作者：
Itsuro Yamane;J. Thomford;Ian A. Gardner;J. P. Dubey;M. Lévy;Patricia A. Conrad
我们确定了吉氏巴贝斯虫间接荧光抗体(IFA)试验中血清学交叉反应的程度，以及试验中血清阳性的最佳截止滴度。在患有自然获得性临床巴贝斯虫病的狗身上检测到的吉伯逊B菌最低滴度为1280，12只狗中有7只的滴度在10,240至20,480之间。两只实验感染的正常脾犬产生了对吉氏杆菌的高滴度(40,960至81,920)，同一血清在IFA试验中对犬弓形虫(滴度=640)、弓形虫(滴度=2,560)和犬新孢子虫(滴度=10,240)起反应。实验感染犬、弓形虫的犬，对吉氏杆菌的滴度为160。实验感染N犬的狗对N犬有80~10,240的滴度，但对吉伯逊B菌没有血清学反应。来自澳大利亚的健康狗的血清学效价为<；或=160。澳大利亚是一个已知不存在吉卜松氏菌的国家。根据这些发现，对于有巴贝斯虫病临床症状的狗的吉伯逊B菌的血清诊断，以320为截止滴度被认为是合适的。更保守的1,280滴度被确定为血清流行病学研究的截止滴度，这是基于假阳性结果的相对成本和假阳性结果的好处，以及在脾切除和免疫抑制后未能从临床正常的狗身上分离出B吉氏寄生虫，其中B吉氏滴度为640。
We determined the extent of the serologic cross-reactivity in the indirect fluorescent antibody (IFA) test for Babesia gibsoni, and the optimal cut-off titer for seropositivity in the test. The lowest titer to B gibsoni detected in a dog with naturally acquired clinical babesiosis was 1,280, and 7 of 12 dogs had titer between 10,240 and 20,480. Two experimentally infected normosplenic dogs developed high titer (40,960 to 81,920) to B gibsoni, and the same sera reacted in IFA tests for B canis (titer < or = 640), Toxoplasma gondii (titer < or = 2,560), and Neospora caninum (titer < or = 10,240). Dogs that were experimentally infected with B canis and T gondii had titer < or = 160 to B gibsoni. Dogs that were experimentally infected with N caninum had titer (80 to 10,240) to N caninum, but failed to have serologic reactivity to B gibsoni. Serologic titer of healthy dogs from Australia, a country where B gibsoni is not known to exist, was < or = 160 to B gibsoni. On the basis of these findings, a cut-off titer of 320 was considered to be appropriate for serodiagnosis of B gibsoni in dogs with clinical signs of babesiosis. A more conservative titer of 1,280 was established as the cut-off titer for seroepidemiologic studies based on relative costs and benefits of false-positive results and failure to isolate B gibsoni parasites after splenectomy and immunosuppression from a clinically normal dog with B gibsoni titer of 640.(ABSTRACT TRUNCATED AT 250 WORDS)