Potato leaf roll virus protein P1 contains a serine proteinase domain
Potato leaf roll virus protein P1 contains a serine proteinase domain
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DOI：
10.1099/0022-1317-81-7-1857
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发表时间：
2000-07-01
影响因子：
3.8
通讯作者：
Lamb, JW
中科院分区：
文献类型：
--
作者：
Li, X;Ryan, MD;Lamb, JW
利用苜蓿银纹夜蛾核型多角体病毒多角体蛋白启动子在昆虫细胞中表达多结构域马铃薯卷叶病毒复制酶蛋白P1。使用抗P1的抗血清，结果表明，P1在昆虫细胞中VPg附近以与植物细胞中相似的方式被切割，产生类似于27 kDa的C-末端片段。此外，它表明，P1内的丝氨酸蛋白酶样结构域是负责这种处理，这可能发生在一个反式（分子间）反应。丝氨酸蛋白酶结构域中的四个保守残基是催化所必需的，已被确定，与该结构域包含丝氨酸蛋白酶的提议一致。
The multi-domain potato leaf roll virus replicase protein P1 was expressed in insect cells from the polyhedrin promoter of Autographa californica nucleopolyhedrovirus. Using antisera raised against P1, it was shown that P1 was cleaved near the VPg in insect cells in a manner similar to that in plant cells, to produce a similar to 27 kDa C-terminal fragment. Furthermore, it was shown that the proposed serine proteinase-like domain within P1 is responsible for this processing and that this can occur in a trans (intermolecular) reaction. Four conserved residues within the serine proteinase domain that are essential for catalysis have been identified, consistent with the proposal that this domain comprises a serine proteinase.