RGD-targeted paramagnetic liposomes for early detection of tumor: In vitro and in vivo studies
RGD-targeted paramagnetic liposomes for early detection of tumor: In vitro and in vivo studies
复制标题
用于肿瘤早期检测的 RGD 靶向顺磁性脂质体：体外和体内研究
DOI：
10.1016/j.ejrad.2011.01.051
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发表时间：
2011-11-01
影响因子：
3.3
通讯作者：
Zhou, Caicun
中科院分区：
文献类型：
--
作者：
Li, Wei;Su, Bo;Zhou, Caicun
在过去的几十年里，磁共振分子成像已经成为一种潜在的肿瘤诊断方法。这种方法包括通过特定的靶向载体将MR造影剂递送到肿瘤。为此目的，通过使用经由KGG三肽间隔物偶联至棕榈酸锚的环状RGD肽头基来构建脂肽。然后通过将RGD偶联的脂肽掺入到脂质双层中以特异性结合肿瘤来制备靶向顺磁性脂质体。体外研究表明，RGD靶向脂质体比非靶向脂质体对靶细胞表现出更好的结合亲和力。与非靶向脂质体和纯造影剂组相比，携带A549肿瘤的小鼠与RGD靶向顺磁性脂质体的MR成像也导致肿瘤的更大信号增强。此外，生物分布研究也显示了RGD靶向顺磁性脂质体在体内的特异性肿瘤靶向性。因此，本研究制备的RGD靶向顺磁性脂质体可能是一种更有前途的肿瘤早期诊断方法。(C)2011爱思唯尔爱尔兰有限公司保留所有权利。
Magnetic resonance molecular imaging has emerged as a potential approach for tumor diagnosis in the last few decades. This approach consists of the delivery of MR contrast agents to the tumor by specific targeted carriers. For this purpose, a lipopeptide was constructed by using a cyclic RGD peptide headgroup coupled to palmitic acid anchors via a KGG tripeptide spacer. Targeted paramagnetic liposomes were then prepared by the incorporation of RGD-coupled-lipopeptides into lipid bilayers for specific bounding to tumor. In vitro, study demonstrated that RGD-targeted liposomes exhibited a better binding affinity to targeted cells than non-targeted liposomes. MR imaging of mice bearing A549 tumors with the RGD-targeted paramagnetic liposomes also resulted in a greater signal enhancement of tumor compared to non-targeted liposomes and pure contrast agents groups. In addition, biodistribution study also showed specific tumor targeting of RGD-targeted paramagnetic liposomes in vivo. Therefore, RGD-targeted paramagnetic liposomes prepared in the present study may be a more promising method for early tumor diagnosis. (C) 2011 Elsevier Ireland Ltd. All rights reserved.