Purification of a novel RECQL5-SWI/SNF-RNAPII super complex.
Purification of a novel RECQL5-SWI/SNF-RNAPII super complex.
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发表时间：
2010
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通讯作者：
Guangjin Zhou;Yifei Liu;Shwu‐Yuan Wu;F. Tie;H. Lou;C. Chiang;G. Luo
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作者：
Guangjin Zhou;Yifei Liu;Shwu‐Yuan Wu;F. Tie;H. Lou;C. Chiang;G. Luo
RecQ解旋酶是进化保守的DNA解旋酶家族的成员。它们与大肠杆菌的RecQ解旋酶同源。大肠杆菌，该家族的创始成员。这些酶包括Bloom、Werner和Rothmund-Thomson综合征中致病基因的基因产物。到目前为止，这些蛋白质已经涉及DNA代谢的许多方面，包括DNA复制，修复和重组。我们在这里报道，RECQL 5，一个新的成员的人RecQ解旋酶家族，物理上相互作用的SWI/SNF复合物和RNAPII核心复合物的背景下，一个超级复杂。RECQL 5在RNAPII全酶中检测到，但在纯化的RNAPII核心复合物中未检测到。总之，这些数据将RECQL 5与RNAPII转录机制的组装联系起来，并表明这种解旋酶可能在RNAPII转录或RNAPII相关过程中具有调节作用。
RecQ helicases are members of an evolutionary conserved family of DNA helicases. They are homologous to the RecQ helicase of E. coli, the founding member of the family. These enzymes include gene products of disease-causing genes in Bloom, Werner, and Rothmund-Thomson syndrome. To date, these proteins have been implicated in many aspects of DNA metabolism, including DNA replication, repair, and recombination. We reported here that RECQL5, a newer member of the human RecQ helicase family, physically interacts with SWI/SNF complex and RNAPII core complex within the context of a super complex. RECQL5 was detected in the RNAPII holoenzyme but not in purified RNAPII core complex. Together, these data link RECQL5 to the assembly of the RNAPII transcription machinery and suggest that this helicase may have a regulatory role in RNAPII transcription or an RNAPII-related process or processes.