Metanephric transforming growth factor-alpha is required for renal organogenesis in vitro.
Metanephric transforming growth factor-alpha is required for renal organogenesis in vitro.
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体外肾器官发生需要后肾转化生长因子-α。
DOI：
10.1152/ajprenal.1992.262.4.f533
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Hammerman,MR
中科院分区：
文献类型：
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作者：
Rogers,SA;Ryan,G;Hammerman,MR
研究了转化生长因子- α (tgf - α)在后肾发育中的作用。从13天大的大鼠胚胎中取出后肾，使用无血清化学定义的培养基在器官培养中生长6天。在此期间，后肾的大小和形态复杂性都有所增加。肾组织中存在tgf - α信使RNA。后肾在体外产生免疫反应性tgf - α，并释放到培养基中。后肾源的tgf - α用重组人125i - tgf - α进行高效液相色谱分离。相反，表皮生长因子未检测到。在培养的4天内，tgf - α的水平相对稳定，平均约为10(-10)m。在器官培养中加入抗tgf - α抗体，可抑制后肾标本的生长、输尿管芽的树突化和后肾囊胚内的小管形成。这些数据证明了大鼠后肾在器官培养中产生tgf - α。这种肽是体外生长发育所必需的。我们的研究结果表明，后肾来源的tgf - α在体内作为肾脏器官发生的促进剂具有必要的作用。
The role of transforming growth factor-alpha (TGF-alpha) in metanephric development was examined. Metanephroi were removed from 13-day-old rat embryos and grown in organ culture for up to 6 days using serum-free chemically defined media. During this time the metanephroi increased in size and morphological complexity. Messenger RNA for TGF-alpha was present in the renal anlage. Immunoreactive TGF-alpha was produced by metanephroi in vitro and released into culture media. TGF-alpha of metanephric origin coeluted with recombinant human 125I-TGF-alpha after high-performance liquid chromatography of media. In contrast, epidermal growth factor was not detectable. Levels of TGF-alpha were relatively constant during 4 days in culture and averaged approximately 10(-10) M. Growth of the metanephric anlage, arborization of the ureteric bud, and tubulogenesis within the metanephric blastema were inhibited by the addition of anti-TGF-alpha antibodies to organ cultures. These data demonstrate production of TGF-alpha by developing rat metanephroi in organ culture. The peptide is necessary for growth and development in vitro. Our findings suggest a necessary role for TGF-alpha of metanephric origin as a promoter of renal organogenesis in vivo.