Lipidomic analysis of Toxoplasma gondii reveals unusual polar lipids.
Lipidomic analysis of Toxoplasma gondii reveals unusual polar lipids.
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弓形虫的脂质组学分析揭示了异常的极性脂质。
DOI：
10.1021/bi7011993
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发表时间：
2007
期刊：
影响因子：
2.9
通讯作者：
McLeod,Rima
中科院分区：
文献类型：
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作者：
Welti,Ruth;Mui,Ernie;Sparks,Alexis;Wernimont,Sarah;Isaac,Giorgis;Kirisits,Michael;Roth,Mary;Roberts,CraigW;Botté,Cyrille;Maréchal,Eric;McLeod,Rima
通过电喷雾电离串联质谱法对弓形虫的极性脂质进行分析，提供了这种寄生原生动物的脂质分子种类的详细图像。最值得注意的是，T.弓形虫含有相对较高水平的神经酰胺磷酸乙醇胺，估计约占总极性脂质的 2%。神经酰胺磷酸乙醇胺具有仅含 16 和 18 个碳原子的脂肪酰胺结构。与生长它的宿主成纤维细胞相比，T.弓形虫还具有较高水平的磷脂酰胆碱，但水平较低的鞘磷脂和磷脂酰丝氨酸。分子物种水平的分析表明T.弓形虫的极性脂质分子种类中比宿主成纤维细胞含有更多的短链脂肪酸。总共有 30 个或更少酰基碳的短链脂肪酸占弓形虫二酰基磷脂酰胆碱的 21%，但仅占宿主二酰基磷脂酰胆碱的 3%。此外，具有两个含 12、14 或 16 个碳的饱和酰基链的二酰基磷脂酰胆碱占寄生虫磷脂酰胆碱的 11% 以上，但占宿主磷脂酰胆碱分子种类的不到 3%。独特的T.弓形虫脂质谱可能特别适合寄生虫膜的功能以及寄生虫与宿主细胞和宿主免疫系统的相互作用。与 T 结合。贡地基因组数据，这些脂质组学数据将有助于阐明这种重要的人类病原体脂质生物合成的代谢途径。
Analysis of the polar lipids ofToxoplasma gondiiby electrospray ionization tandem mass spectrometry provides a detailed picture of the lipid molecular species of this parasitic protozoan. Most notably,T. gondiicontains a relatively high level, estimated to about 2% of the total polar lipid, of ceramide phosphoethanolamine. The ceramide phosphoethanolamine has a fatty amide profile with only 16- and 18-carbon species. Compared with the host fibroblasts in which it was grown,T. gondiialso has higher levels of phosphatidylcholine but lower levels of sphingomyelin and phosphatidylserine. Analysis at the molecular species level indicated thatT. gondiihas greater amounts of shorter-chain fatty acid in its polar lipid molecular species than the host fibroblasts. Shorter-chain fatty acids with a combined total of 30 or fewer acyl carbons make up 21% ofToxoplasma's, but only 3% of the host's, diacyl phosphatidylcholine. Furthermore, diacyl phosphatidylcholine with two saturated acyl chains with 12, 14, or 16 carbons make up over 11% of parasite phosphatidylcholine but less than 3% of the host phosphatidylcholine molecular species. The distinctiveT. gondiitachyzoite lipid profile may be particularly suited to the function of parasitic membranes and the interaction of the parasite with the host cell and the host's immune system. Combined withT. gondiigenomic data, these lipidomic data will assist in elucidation of metabolic pathways for lipid biosynthesis in this important human pathogen.