A method for determination of low carbon monoxide concentration in blood.
A method for determination of low carbon monoxide concentration in blood.
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一种测定血液中低一氧化碳浓度的方法。
DOI：
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发表时间：
1966
期刊：
影响因子：
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通讯作者：
B. Söderström
中科院分区：
文献类型：
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作者：
H. Linderholm;T. Sjöstrand;B. Söderström
描述了一种测量血液中一氧化碳含量（特别是低浓度时）的方法。单次测定的误差为分析样品中CO总量（范围0.7-70 μl）的1- 3%。在约0.1 ml CO/100 ml血液的普通CO含量下，误差约为± 0.002 ml CO/100 ml血液（S.D.）。比较了从血液中释放CO的不同程序。与K3 Fe（CN）6和H2SO 4相等，略小于与KH（JO 3）2的CO量，如果血液与低CO含量进行了分析。显然，KH（JO 3）2虽然比其他化学试剂（K2 Cr2 O 7和NaOH）少，但会导致血液中额外的CO形成。尽管由于这种额外的CO形成的误差，但当这种小误差变得微不足道时，使用KH（JO 3）2在更高范围的CO浓度下进行分析是有利的。如果从血液中提取的CO与试剂，如K3 Fe（CN）6，H2SO 4或KH（JO 3）2发生在一个高的O2分压略大于CO的量比在“真空”或在N2的气氛中提取获得。结果表明，在用于从血液中释放CO的过程中可能会形成少量的CO，这可能是由于血红蛋白的氧化分解。
A method is described for the measurement of the CO content of blood, particularly at low concentrations. The error of a single determination was 1–3 per cent of the total amount of CO (range 0.7–70 μl) present in the analyzed sample. At an ordinary CO content of about 0.1 ml CO/100 ml blood the error was about ± 0.002 ml CO/100 ml blood (S.D.). Different procedures for the release of CO from the blood were compared. With K3 Fe(CN)6 and H2SO4 equal and slightly smaller amounts of CO were obtained than with KH(JO3)2, if blood with a low CO content was analyzed. Evidently KH(JO3)2, although less than some other chemical agents (K2Cr2O7 and NaOH), causes extra CO formation from the blood. In spite of the error due to this extra CO formation there were advantages to use KH(JO3)2 for analysis in higher ranges of CO concentrations, when this small error became insignificant. If extraction of CO from the blood with reagents such as K3 Fe(CN)6, H2SO4 or KH(JO3)2 took place at a high O2 partial pressure slightly larger amounts of CO were obtained than with extraction in“vacuum” or in an atmosphere of N2. The results indicate that small amounts of CO may be formed during procedures used for the release of CO from blood, probably due to oxidative break down of hemoglobin.